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Sandringham Road, Southport PR8 - Average value [pounds sterling]1,053,977
Mice were inoculated intranasally with a single, nonlethal dose of the IAV PR8 strain (300 pfu).
A dense concentration of PR8 ([10.sup.9] TCID50/ml) was incubated with medium only or pretreated with 400 [micro]g/ml maoto for 0.5 min or 60 min and then was used to infect A549 cells (moi = 1) followed by the culturing (maoto concentration < 1 [micro]g/ml).
Handwritten images Machine printed images Image Resolution Fill Image Resolution Fill factor factor HW1 645 x 743 12.7% PR1 1381 x 368 16.8% HW2 1218 x 781 4.6% PR2 1180 x 371 11.7% HW3 1870 x 511 7.5% PR3 1203 x 363 18.4% HW4 469 x 597 9.3% PR4 1838 x 798 11.3% HW5 1623 x 261 11.2% PR5 690 x 682 13.8% HW6 787 x 687 7.3% PR6 1315 x 1069 5.0% HW7 982 x 657 4.1% PR7 600 x 564 2.5% HW8 998 x 410 4.8% PR8 859 x 232 13.8% TABLE II.
k0602 Re-4/Re-5 1/7 rk0602 10/10 Control 0/5 ([double dagger]) k0603 Re-4/Re-5 1/10 rk0602 10/10 Control 0/5 ([double dagger]) * Chickens were immunized with the Re4/Re5 or the inactivated rk0602 vaccine (the HA and NA genes of rk0602 were derived from subtype H5N1 k0602 virus; the internal genes were from PR8), and HI antibody titers were determined on day 28 postvaccination.
The median infectious dose ([ID.sub.50]) of PR8 in mice was calculated by the method of Reed and Muench (1938).
Write to 10 Portland Street, PR8 1LJ or email firstname.lastname@example.org to be removed from the mailing list.
The second technique involves injecting both the H1N1 and PR8 viruses into eggs and allowing the hybrid strain to be created through a natural re-assortment of their genes.
Call 0845 603 7788, click on www.gro.gov.uk or write to Family Records Centre, Overseas Section, General Register Office, Smedley Hydro, Trafalgar Road, Birkdale, Southport, Merseyside PR8 2HH.
For further clarification, write to the General Register Office, Smedley Hydro, Trafalgar Road, Birkdale, Southport PR8 2HH.
Wild-type influenza A viruses A/Puerto Rico/8/34 (PR8, H1N1; laboratory strain) and A/Singapore/1/57 (Sg, H2N2; ATCC) were propagated in embryonated chicken eggs.
CONTACT: Alan Wright, 57 Linaker Street, Southport, PR8 5DQ, 01704 537817.
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