Lack of PTEN
expression in endometrial intraepithelial neoplasia is correlated with cancer progression.
But armed with the new genetic insights regarding PTEN
The membranes were probed against PTEN
(Santa Cruz, USA), or cleaved caspase-3 (Cell Signaling Technology, USA), and reprobed against GAPDH (Santa Cruz) as a loading control.
U6 and [beta]- actin were used as internal controls for miR-21 and PTEN
mRNA quantitation, respectively.
expression score was calculated as the product of the staining intensity and the percentage of positive tumor cells.
DNA ANALYSIS FOR PTEN
AND STK11 MUTATIONS BY MULTIPLEX LIGATION-DEPENDENT PROBE AMPLIFICATION
Previous studies had linked mutations in PTEN
with the unchecked growth of cancer cells.
Whites were more likely to have the PTEN
mutation (17 of 78 patients, or 22%), compared with African Americans (3 of 62 patients, or 5%), said the study's lead investigator, Dr.
The researchers stained the samples for PTEN
expression and linked that data with disease-free survival.
In this study, we demonstrated that PTEN
downregulation and resultant activation of PI3K signaling caused PKC[zeta] activation, which in turn increased ROS production through NADPH oxidase expression and its activity modulation in replicative aged human dermal fibroblasts (HDFs).
The E3 ubiquitin ligase neural precursor cell expressed developmentally down-regulated 4-1 (NEDD4-1) negatively regulates PTEN
protein stability through polyubiquitination.
gene mutations defined in approximately 60% of the patients and the disorder is classified as one of the PTEN
Hamartomatous Tumor Syndromes.