In addition to the conferences, fellows also attended at least one ItAG or PTLC
meetings of their choosing on topics such as teaching about racial justice in the wake of the events in Ferguson, Missouri, standardized testing, and hip hop in the classroom.
The work of the PTLC
testifies to the fact that, even in the inner city where the pressure of standardized testing is especially high (Meier & Wood, 2004), the progressive, child-centered legacy in education still continues.
transplant recipients who received lungs with a pTLC
ratio of 1.0, a
officinalis Nauclea officinalis AD Alzheimer's disease ACh acetylcholine ChEIs cholinesterase inhibitors C[H.sub.2][Cl.sub.2] dichloromethane MeOH methanol DEB dichloromethane extract of bark DEL dichloromethane extract of leaves MEL methanol extract of leaves HPLC high performance liquid chromatography PTLC
preparative thin layer chromatography TcAChE Torpedo californica acetylcholinesterase hBChE human butyrylcholinesterase LB Lineweaver-Burk [K.sub.i] inhibition constant ARTICLE INFO
F2/2-3/1-F2/2-3/10), which were separated using pTLC
(solvent-toluene-EtOAc-HCOOH 6: 3: 1) to give umbelliferone (12; 6 mg), aesculetin (13; 14mg) and scopoletin (16; 5mg) .
Preparative thin layer chromatography (PTLC
) was performed on aluminum oxide plates (20 x 20 cm) of 250 um thickness.
These fractions were purified by PTLC
using different solvent systems.
Sixty-one isolates of Penicillium crustosum originating from various foodstuffs were screened for penitrem A (15) production by high- PTLC
. The highest producers of penitrem A (4 isolates) were grown in various liquid media.
After identification of the inhibition zones on the TLC plate, PTLC
was performed by loading the essential oil onto a preactivated silica gel 60 [F.sub.254] coated glass plate (20 x 20 cm, 500 [micro]m thickness) which was developed in n-hexane/ethyl acetate (9:1, v/v) solvent system.
The major compound was further purified by PTLC
using solvent system n-hexane-acetone (0.8:9.2) afforded compound 2 (13 mg), Rf 0.33.
Seven components (E1-E7) were isolated, among which components E2, E3, E4, and E5 for their large quantity were further purified by Sephadex LH-20, PTLC
, Amberlite XAD2, and recrystallization and 5 compounds were obtained and their structures were identified by IR (Nicolet impact 400), MS (HP 5988A GC/MS), and 1D NMR and 2D NMR (Bruker-500 MHz-FT-NMR).
To isolate the alkaloids we used silica gel 60 [F.sub.254nm] as stationary phase in a preparative thin layer chromatography (PTLC
) and CH[Cl.sub.3]:C[H.sub.2][Cl.sub.2]: ethyl acetate:Me0H 4.5:4:1:0.5 (v/v) as eluents in an ammonium hydroxide (N[H.sub.44]OH) atmosphere (40 mg of CE/per plate), which led to isolate two indole alkaloids, ramiflorine A and ramiflorine B, already known and described in stem barks, their structures were established by uni-and bidimensional nuclear magnetic resonance (NMR) and mass spectrometry (MS) data by comparison with literature values (Marques etal.