Yeast(s) DV10 EC1118 YAN (ppm) 225 200-350 DAP/nutrient additions Yes DAP as needed and Thiamine @ 58mg/hL Fermentation temperature 55[degrees] to 60.8[degrees] F 60[degrees] F Fining Polyvinylpoly- Isinglass post pyrrolidone (PVPP
) fermentation, followed by cellulose filtration Racking Yes 24 hours after pressing, and post fermentation.
was purchased from Gobekie New Materials Science & Technology Co.
column was used in the present study to adsorb polyphenols and the remaining solution passing through the column was collected to study the contribution of polyphenols to the bioactivity of natural products.
The adaptation consisted of replacing the membrane filter (higher diameter and light mesh) and the extraction buffer (CTAB + PVPP
), increasing the number of filters (two filter membranes for each 10 L sample (5 L each)) and three extractions for each membrane filter.
CCNa, L-HPC, PVPP
and CMS-Na were selected as superdisintegrants.
is permitted for use in beverages in all countries with regulations covering the use of additives and processing aids.*
En el proceso de estabilizacion se hablo sobre los distintos tipos de agentes estabilizantes: polivinilpolipirrolidona (PVPP
), silica gel (SG) y la combinacion de ambos para optimizar el proceso [15,16].
The composite samples were homogenized on ice (2% PVPP
was added to the homogenates of young fruits before days 90 after full bloom) and then centrifuged at 4,000xg for 15 min at 4[degrees]C.
La extraccion de proteinas se llevo a cabo pesando 0.3 g del polvo acetonico anteriormente preparado y poniendolo en contacto con 25 ml de buffer Tris pH 8 (Tris 100 mM, EDTA 1 mM, PVPP
0,6 mg/100 ml), al que se le adicionaron 200 [micron]l del inhibidor de proteasas fluoruro de fenilmetilsulfonilo (PMFS) 1 mM.
Prior to evaporation, the methanolic extract was purified by treating with 1g of polyvinylpolypyrolidone (PVPP
) at 0[degrees]C for 30 min and Solid Phase Cartridge (Strata-x-33 [micro]m polymeric sorbent, 8B-S100-FCH-S, Phenomenex, Aschaffenburg,
(1993) for determination of non-tannin phenolics, 100 mg PVPP
was weighed in test tubes before being added with 1.0 mL distilled water and 1.0 mL of the extracted sample .
For instance, cetyl trimethylammonium bromide (CTAB) buffers including a reduction agent such as P-mercaptoethanol have been tested alone or in combination with either polyvinylpyrrolidone (PVP), or polyvinylpolypyrrolidone (PVPP
) (STADDON et al., 2004; TURRINI et al., 2008).