Table-1 shows the dependence of the amount activity and stability of immobilized trypsin from the molecular weight of PVPr, the nature of the metal ion and the mass (%) of PVPr in graft copolymer composition which is obtained by reacting VPr with AG from the cherry gum.
Table-1 Influence of the molecular weight of PVPr and mass (%) VPr grafted on AG and the nature of the metal ions the activity and the amount of immobilized trypsin.
PVPr M n =4A-10 4###0.45###22.50###51###7.42###11.68
This is due to the fact that increasing the sorption amount and swelling degree of gel obtained by cross-linking high molecular PVPr is more easily to introduce the protein in macromolecules hydrogels.
Interaction of trypsin with PVPr confirms the data by NMR spectroscopy.
As seen from Table-1 the amount of activity and stability of immobilized trypsin gel-based carrier with a molecular weight PVPr 4A-104 are of less value than immobilization trypsin to Cu(II), Co(II) and Ni(II) complexes a PVPr gel, which were obtained after the sorption of metal ions.
This may explain the process involving metal ions coordinated to PVPr gels trypsin immobilization process.
PVPr complexes with metal ions [MeX2A-2L]n, give the shift in at 1670 cm-1 due to the carbonyl group in the pyrrolidone ring.