Prediction of retention in gradient reversed-phase liquid chromatography for phenylisothiocyanate
derivatives of amino acids.
Nevertheless, DLM method results for glatiramer acetate (thiol-free peptide), used as a negative control, revealed that fluorescence response was only reduced at placebo-response levels after phenylisothiocyanate
titration against its amine groups (Table 5).
Calbucci et al., "High-performance liquid chromatographic analysis of physiological amino acids in human brain tumors by pre-column derivatization with phenylisothiocyanate
," Journal of Chromatography B, vol.
The active methylene moiety of 2-cyano-N'-[1-(4-methoxyphenyl)ethylidene]acetohydrazide (3a) allowed reacting with phenylisothiocyanate
in dry N, N-dimethylformamide DMF containing catalytic amount of potassium hydroxide  yielding the nonisolable intermediate potassium sulfide salt 12, and then ethylchloroacetate was added affording 1,3-thiazolidinone derivative 14.
The samples were initially subjected to automatic pre-column derivatization with a phenylisothiocyanate
After filtration, 25 [micro]l were used for derivatization with phenylisothiocyanate
and derivatized samples were analyzed in a Waters Nova-pak C18 column (5[micro]m, 3.9x150mm).
Next, 7:1:1:1 ethanol: TEA: water: phenylisothiocyanate
were added and reactions proceeded for 20 min under NZ atmosphere.
We derivatized the free amino groups of PBG and ALA with phenylisothiocyanate
(PITC; Pierce) by adding 50 [micro]L of PITC reagent (ethanol-water-triethylamine-PITC, 7:1:1:1 by volume) to each dried sample to form phenylthiocarbamyl-PBG (PTC-PBG) and phenylthiocarbamyl-ALA (PTC-ALA), respectively.
Free amino acids in soluble N fractions were derivatized with phenylisothiocyanate
and separated and quantified using reversed-phase high performance liquid chromatography (Heinrikson and Meredith, 1984).
In our previous work, a HPLC method was developed and validated for the simultaneous determination of twenty AAs in QKLI based on a pre-column derivatization with phenylisothiocyanate
Improved HPLC determination of acidic opines by phenylisothiocyanate
derivatization and its application to marine animals.
This system uses a biotin-labeled oligonucleotide probe detected with an avidin-biotin phenylisothiocyanate
system for signal amplification.