Prediction of retention in gradient reversed-phase liquid chromatography for phenylisothiocyanate
derivatives of amino acids.
Cohen SA and DJ Strydom Amino acid analysis utilizing phenylisothiocyanate
Next, 7:1:1:1 ethanol: TEA: water: phenylisothiocyanate
were added and reactions proceeded for 20 min under NZ atmosphere.
We derivatized the free amino groups of PBG and ALA with phenylisothiocyanate
(PITC; Pierce) by adding 50 [micro]L of PITC reagent (ethanol-water-triethylamine-PITC, 7:1:1:1 by volume) to each dried sample to form phenylthiocarbamyl-PBG (PTC-PBG) and phenylthiocarbamyl-ALA (PTC-ALA), respectively.
Free amino acids in soluble N fractions were derivatized with phenylisothiocyanate
and separated and quantified using reversed-phase high performance liquid chromatography (Heinrikson and Meredith, 1984).
derivatization and liquid chromatography of amino acids in food.
Improved HPLC determination of acidic opines by phenylisothiocyanate
derivatization and its application to marine animals.
This system uses a biotin-labeled oligonucleotide probe detected with an avidin-biotin phenylisothiocyanate
system for signal amplification.
Cystine was determined as cysteic acid and methinonine as methionine sulfone after preoxidation with performic acid and pre-column derivation using phenylisothiocyanate
(L-8800 Hitachi Automatic Amino Acid Analyzer, Tokyo, Japan).
Amino acids in CSF can be determined either by postcolumn derivatization with ninhydrin or o-phthalaldehyde (OPA) by commercially available amino acid analyzers or by precolumn derivatization with different reagents, such as dansyl chloride, phenylisothiocyanate
, fluorenylmethyl chloroformate, dabsyl chloride, and OPA, followed by HPLC separation (4-6).