Total RNA was extracted from liver and heart tissues, reverse transcribed and quantified to evaluate the expression of the cardiac and liver Troponin (TNT) and Phospholamban
2+] exchanger PLB: Phospholamban
ROS: Reactive oxygen species RyR: Ryanodine receptor SERCA: Sarco-/endoplasmic reticulum [Ca.
Ketzer LA, Arruda AP, Carvalho DP, de Meis L (2009) Cardiac sarcoplasmic reticulum Ca2+-ATPase: heat production and phospholamban
alterations promoted by cold exposure and thyroid hormone.
And the researchers found BPS and BPA had similar effects in quickly and temporarily increasing the phosphorylation of calcium-handling proteins, including ryanodine receptor and phospholamban
Conformational changes within the cytosolic portion of phospholamban
upon release of Ca-ATPase inhibition.
Semi-quantitative RT-PCR was used to assess changes in phospholamban
gene expression in treated and untreated rats.
It started when members of Medical scientist Howard Young's team, Delaine Ceholski and Cathy Trieber, discovered a new mutation in a protein called phospholamban
, which they predicted would cause the heart to be less responsive to changes in the body and eventually lead to heart failure.
It is notable that many of the genes identified by GWASs for these subphenotypes of cardiac or electrical conductance include those for ion channels or proteins that regulate the intracellular concentrations of electrolytes, such as phospholamban
8,29) Some of the commonly mutated genes causing the AD type of familial DCM include lamin A/C, betamyosin heavy chain, alphamyosin heavy chain, actin, alpha-actinin-2, metavinculin, desmin, sarcoglycan, troponin T, alpha-tropomyosin, titin and phospholamban
Measurements have included L-type calcium transient using the voltage patch clamp technique, phosphorylation of signalling proteins cAMP, cGMP and phospholamban
concentrations, PDE isoform inhibition and calcium transient amplitude using aquorin immunoflurescence (57,88,92).
The main regulator of SERCA2a is phospholamban
and another regulator proteolipid of SERCA is sarcolipin.