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The pseudo-maximum-likelihood method described in Wang (2001) was used to obtain estimates and 95% confidence intervals of [N.sub.eV] by using the program MLNE available at http://www.zoo.cam.ac.uk/ ioz/software.htm#MLNE.
Values of C and G obtained for each locality were subsequently used to correct estimates of [N.sub.e] by [N.sub.ec] = [N.sub.e] x [C/G], where [N.sub.e] is the pseudo-maximum-likelihood estimate of variance effective size obtained by following Wang (2001).
The pseudo-maximum-likelihood (temporal-method) estimates of variance effective size ([N.sub.eV]), corrected for overlapping generations, and their 95% confidence intervals for all three localities are shown in Table 4.
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