Q-PCR

Acronym	Definition
Q-PCR	Quantitative Competitive Polymerase Chain Reaction

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Quantitative real-time PCR (Q-PCR) was performed using an ABI Prism 7500 sequence-detection system (Applied Biosystems, Foster City, CA, USA) with SYBR Green Realtime PCR Master Mix (Toyobo, Osaka, Japan), under the manufacturer's instructions.

Gene expression and promoter methylation of porcine uncoupling protein 3 gene

Table I.- Primer information of candidate gene using q-PCR.

Identification of Differentially Expressed Long Noncoding RNAs and mRNAs Involved with Dominant Follicle Selection in Goats using RNA-seq

To validate gene expression, we randomly chose the upregulated genes HIF-1A, NF-[kappa]B, and BIRC3 and the downregulated gene Bcl2 to do the q-PCR. The specificity of q-PCR primers was confirmed by regular PCR (Additional file 3).

Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs

The Q-PCR experiment was performed in triplicate, with each reaction solution (20 [micro]L) containing 200 nM of each primer, 10 [micro]L of 2X SYBR Green Supermix (BioRad), and 5-30 ng of the DNA template.

Community and Proteomic Analysis of Anaerobic Consortia Converting Tetramethylammonium to Methane

Absolute Q-PCR. To generate the absolute quantitative standard curve for Q-PCR analysis on lentivirus copy number measurement, we used the PCR product of the mouse GUS gene and cloned it with the TA cloning vector (pTA[R] Easy Cloning Kit), which was purchased from Genomics BioSci and Tech (Taipei, Taiwan).

CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer

(a) Expression of Cadm1 in different developmental stages of mouse inner ear examined by Q-PCR. The bar graphs show quantification of the PCR results with each value representing the mean [+ or -] standard error.

Identification of Binding Partners of Deafness-Related Protein PDZD7

Expression of IL-10 and TLR4 in T Cell after Transfection by Q-PCR and ELISA.

Regulatory Role of rno-miR-30b-5p in IL-10 and Toll-like Receptor 4 Expressions of T Lymphocytes in Experimental Autoimmune Uveitis In Vitro

Moreover, quantitative analysis using q-PCR revealed that HDF supplemented with 30 [micro]M RA suppressed mRNA expression of iNOS to 20.8% of TNF-[alpha] alone (P<0.05; Figure 3B).

Effect of a rosmarinic acid supplemented hemodialysis fluid on inflammation of human vascular endothelial cells

The Ultrasensitive Q-PCR Assay for EGFR Mutation Detection

Analytic and Clinical Validation of an Ultrasensitive, Quantitative Polymerase Chain Reaction Assay for EGFR Mutation Analysis With Circulating Tumor DNA

Table 1: Gene-specific primers used in Q-PCR analysis

Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

In order to investigate the effect of mechanical injury on ECM metabolism, the ECM production of L929 fibroblast was detected using Western blot and q-PCR after 5333 [mu]strain.

Mechanism of Mechanical Trauma-Induced Extracellular Matrix Remodeling of Fibroblasts in Association with Nrf2/ARE Signaling Suppression Mediating TGF-[beta]1/Smad3 Signaling Inhibition

The authors would also like to thank the Genetics and Molecular Biology Laboratory, Directorate of Poultry Research, Hyderabad, for giving them access to their Q-PCR facility.

Identification of NURR1 (Exon 4) and FOXA1 (Exon 3) Haplotypes Associated with mRNA Expression Levels in Peripheral Blood Lymphocytes of Parkinson's Patients in Small Indian Population