Q-PCRQuantitative Competitive Polymerase Chain Reaction
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Q-PCR analysis revealed no significant difference in the mRNA levels of OX1R [Figure 4]a and 4b and OX2R [Figure 4]c and 4d in the hypothalamus or the brain stem between the SOD1-G93A transgenic mice and controls.
The Q-PCR exper vents were performed in a 7500 Fast Real-Time PC] ystem (Applied Biosystems) using a hot-start version c nodified Tbr DNA polymerase with SYBR Green I flue escent dye and ROX passive reference dye (DyNAmo HSYBR Green qPCR kit, Finnzymes, Espoo).
By relative Q-PCR, the expressions of chains were compared on three different days after starting transfection:
We performed Q-PCR to validate the microarray data.
However, the Q-PCR could not distinguish between M gallisepticum and Mycoplasma imitans.
However, in developing the next generation of BioWatch, HSARPA has funded a broad range of technologies and platforms, including microarrays, Q-PCR and microfluidics technologies.
Q-PCR was carried out using the SYBR(R) Premix Ex TaqTM (Perfect Real Time) (TaKaRa Japan).
Q PCR was performed by targeting the nonpolymorphic Epstein-Barr nuclear antigen-1 (EBNA-1) gene using Corbett Research 6000 Q-PCR instrument and Rotor gene 6000 software.