The consequences of qualifying as a QHT are that the hedge is ignored and the economic package, consisting of the two legs of the hedging transaction, is treated as a synthetic debt instrument denominated in the foreign currency that the taxpayer pays (under a liability hedge) or receives (under an asset hedge).
If the QHT is a synthetic liability, the interest expense would be subject to allocation and apportionment under the Sec.
After primary concentration-response screening (by qHTS
) (Attene-Ramos et al.
The qHTS ARE beta lactamase reporter gene assay (ARE-b/a) can detect compounds that activate the ARE pathway and induce oxidative stress (Attene-Ramos et al.
Despite the substantial data obtained from HTS and/or qHTS studies, the relationship between in vitro and in vivo toxicity remains unclear (Low et al.
To identify environmental chemicals and drugs that might affect mitochondria dysfunction, we used a qHTS
approach to evaluate the compounds from the Tox21 10K compound library that potentially reduce the mitochondrial membrane potential (MMP) in human liver carcinoma (HepG2) cells.
The 10K library is being screened three times in each qHTS
assay at the NCGC, with compounds in a different well location during each run, to better evaluate assay reliability and to increase the ability to distinguish between weak active and inactive compounds.
2008) is leading the field in exploring how a broad spectrum of in vitro assays, many in qHTS
format, can be used to screen thousands of environmental chemicals for their potential to disturb biological pathways that may result in human disease (Xia et al.
The performance of the proposed algorithm was evaluated with simulated qHTS
KEY WORDS: ARE, Nrf2, oxidative stress, qHTS
, toxicity, Tox21.
METHODS: We examined the data from the Tox21 pilot-phase collection of approximately 3,000 environmental chemicals profiled in qHTS
format against a panel of 10 human nuclear receptors (AR, ER[alpha], FXR, GR, LXR[beta], PPAR[gamma], PPAR[delta], RXR[alpha], TR[beta], and VDR) for reproducibility, concordance of biological activity profiles with sequence homology of the receptor ligand binding domains, and structure--activity relationships.
OBJECTIVES: Our goal was to test a hypothesis that dose--response data points of the qHTS
assays can serve as biological descriptors of assayed chemicals and, when combined with conventional chemical descriptors, improve the accuracy of quantitative structure--activity relationship (QSAR) models applied to prediction of in vivo toxicity end points.