QNRCQuality of Natural Resources Commission (Gastonia, NC)
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For each primer (QnrA, QnrB, QnrC, QnrD, QnrS) amplification was executed as: single initial denaturation and bacterial lysis at 95AdegC for 10 minutes, followed by 35 cycles of denaturation at 95AdegC for 40 seconds, annealing temperature for QnrA, QnrB was 54AdegC, QnrC 50AdegC, QnrD 52AdegC, and QnrS 51AdegC, elongation at 72AdegC for 40 seconds followed by single final elongation at 72AdegC for 10 minutes.
Qnr allele frequencies were found to be 3(3.6%), 9(10.8%), 0%, 5(6%) and 2(2.4%) for QnrA, QnrB, QnrC, QnrD and QnrS respectively.
For this purpose, 256 isolates were tested against QnrA, QnrB, QnrC, QnrD and QnrS genes.
pneumoniae isolates that were resistant to ciprofloxacin and/or levofloxacin were screened for 5 quinolone resistance proteins (qnrA, qnrB, qnrC, qnrD, and qnrS) and one quinolone-modifying enzyme, acc(6')-Ib-cr.
The gene encoding Qnr protein detected least often was qnrC: only one isolate tested positive; qnrA was not detected.
We found that qnrC was represented by only a single isolate, which was consistent with findings from the recent Turkish and Tunisian studies that failed to detect qnrC among quinolone-resistant K.
New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis.