Although DNA markers like AFLPs and microsatellites are preferred for genetic diversity study due to their informativeness, Randomly Amplified Polymorphic DNA (RAPD
) analysis has also been broadly employed to analyze the genetic diversity because it has a universal set of primers, no prior work such as probe isolation, or nucleotide sequencing is necessary (Hadrys et al., 1992).
makers (n=8) produced 245 bands (30.62+-2.87 Mean+-SE / primer) of different molecular weights (126-3342 bp), of which, 96 were population specific.
Prism Red to identify genetic markers linked to salt tolerance in in vitro callus cultures using RAPD
and ISSR markers.
is done by picking one or more short primers of "random" sequence because it's not quite random; homopolymer runs are a bad idea, and some particular sequences may be known to occur repetitively throughout a genome, so you'd ideally avoid both.
The male specific bands were generated through the comparative study of male and female plants using RAPD
The amplified RAPD
product was separated by electrophoresis in 1.5% agarose gel with 1 X TAE buffer, stained with ethidium bromide (0.5 [micro]g/ml) at 90 V for 1.0 to 1.5 hrs.
Genetic similarity matrix based on Nei and Li's index revealed similarity coefficients ranging from 92-97% indicating lower level of genetic polymorphism revealed by RAPD
KEYWORDS: Durum wheat accessions, genetic diversity, ISSR, RAPD
In this study, it was used multiple regression analysis to identify associations between ISSR and RAPD
markers with some agronomic traits in lemon balm ecotypes.
In contrast, primer 2 and primer 3 (OPT 07 and OPH 09) were least informative and they produced mostly undistinguishable banding profiles between the amplified samples of each group after RAPD
assays as shown in Figure 3.
Therefore, present study was carried out to identify the cause of the disease (Koch's postulates), molecularly identify and characterize root pathogens of arid legumes using RAPD
and ITS rDNA polymorphism to reveal inter and intra specific genetic relationships among and within root pathogens, to facilitate effective management of major diseases.