This study aimed to detect the RAPD PCR fingerprint, antibiotic resistance patterns, and the presence of armA gene in clinical KPC-producing isolates and nonproducing isolates of K.
RAPD PCR. Genetic analysis of RAPD showed 30 distinct patterns from D1 to D30, as 5 distinct clusters (Figure 1).
fimicola isolates were subjected to RAPD PCR
to determine the genetic relatedness and genetic diversity (Table 4).
(2005) used RAPD PCR
using 14 arbitrary primers to differentiate 8 strains.
In RAPD PCR
analysis more polymorphism was observed using primers OPA 2, OPA 4,
Several authors (Welsh and McClelland, 1990; Bardakci and Skibinski, 1994; Naish et al., 1995) have demonstrated that the RAPD PCR
method is a powerful tool for the assessment of genetic markers that are capable of discriminating between species or subspecies in a wide range of organisms, including fishes.
Parentage analysis using RAPD PCR
. Nucleic Acids Res.
RAPD PCR analysis: Random Amplified Polymorphic DNA analyses were done using random decamer primers synthesized by Genelink Company, USA.
Gut content analysis of the insect predator species by RAPD PCR molecular technique showed presence of a similar unique DNA fragment in fed H.
analysis using arbitrary primers can be less repeatable than PCR amplification from more specific primers.
RAPD PCR reactions, modified from Skroch and Nienhuis (1995), consisted of 1.25 ng/[micro]L genomic DNA, 0.2 mM primer, 0.4 mM of each dNTP, 0.1 U Taq DNA polymerase (Promega, Madison, WI), 2.5 mM Mg[Cl.sub.2], 50 mM KCl, 10 mM Tris-HCl (pH 8.4), 0.1 mg/mL BSA, 1% (w/v) Ficoll 400 and 0.001% (w/v) xylene cyanol.
The RAPD PCR amplification was performed using a GeneAmp PCR System 2400 (Perkin Elmer, Norwalk, CT).