reaction was performed according to the protocol of Williams et al.
fimicola isolates were also subjected to RAPD-PCR
by 8 RAPD primers for initial screening.
Typing of Lactobacillus sanfranciscensis isolates from traditional Sourdoughs by combining conventional and multiplex RAPD-PCR
Their results suggest that RAPD-PCR
is a useful method for analyzing genetic variation within and between populations of Fusarium oxysporum f.
Bacillus cereus isolates obtained from milk samples were submitted to RAPD-PCR
according to the method described by NILSSON et al.
Therefore, optimization of PCR conditions, that include reagents, temperature, number of cycles and other parameters are very necessary to get a successful RAPD-PCR
Population genetics with RAPD-PCR
markers: Aedes aegypti in Puerto Rico.
So, it appears that RAPD-PCR
may be the most suitable procedure for typing LAB isolates belonging to different genera and species obtained from some fermented foods, such as goat and Manchego cheeses, Almagro eggplant and Tempranillo wine.
Up to now, no results have been published on caraway DNA analysis by RAPD-PCR
, as well as by other molecular markers.
Table 1 Primers and primer sequences used in the RAPD-PCR
Cobb BD, Clarkson JM (1993) Detection of molecular variation in the insect pathogenic fungus Metarhizium using RAPD-PCR
La separacion de los productos de RAPD-PCR
se realizo en geles de agarosa 3%, corridos durante 2,5 horas a 80 V y tenidos con bromuro de etidio.