amplification products comprised different bands (fig 2,3, and 4), table-6 cleared that most efficient and highest discriminatory power was 35.4% and 37% respectively for the primer OPA-10, also the cluster analysis based on RAPD-PCR
products showed two divergent groups with 15% similarity, the first group contained all salt sensitive isolates, while the second group included all salt moderate and tolerant isolates, this group subdivided into two subgroups with 44% similarity, the first subgroup included all salt tolerant isolates which show 100% similarity between them, and the second subgroup comprised the salt moderate tolerant.
Genetic diversity and identification of some Turkish cotton genotypes (Gossypium hirsutum L.) by RAPD-PCR
analysis for DNA extraction, strains were grown overnight in MRS broth (Himedia, Mumbai, India).
aureus isolates was determined by RAPD-PCR
genotyping using four different primers (Table 2).
(25) From this suspension, 2 [micro]l were withdrawn for the RAPD-PCR
and ERIC-PCR reactions in order to analyze genetic variability.
Based on the RAPD-PCR
amplification profiles generated by R3 primer, a cluster dend rogram was constructed (Fig.
In the present study, RAPD-PCR
technique showed that causative agent was L.
DNA Fragmentation and Genetic Analysis Using RAPD-PCR
. Six of 10-mer primers were used for investigating the significant changes of the DNA isolated from brain tissues which was loaded on a 1.5% agarose gel.
Their results suggest that RAPD-PCR
is a useful method for analyzing genetic variation within and between populations of Fusarium oxysporum f.
In this study, rapd-pcr
technique was used for identification of dna polymorphism and changes of genetic structure of catfish "Clarias Gariepinus" as result of environmental pollutions rapd markers showed a high level of polymorphism and a high number of clearly amplified bands.
Population genetics with RAPD-PCR
markers: Aedes aegypti in Puerto Rico.
A set of 92 strains of lactic acid bacteria (LAB) and 10 type-reference strains were analyzed using three molecular techniques: the randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR
); the enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR); and the polytrinucleotide-polymerase chain reaction (GTG)5-PCR).