We used methylated DNA probes to capture methylated targets, which were oligonucleotides from the promoter regions of cancer-related genes (cadherin 1 [ CDH1 ], and retinoic acid receptor beta [ RARB ]) as a detection model.
Probe and target sequences in CDH1 and RARB are shown in [Table 1].
DAPK1, MGMT and RARB
promoter methylation as biomarkers for high-grade cervical lesions.
Finally we also analyzed the genes for retinoid receptors RARB and RXRG, which were found to be methylated in many types of cancer .
3) -- HLTF Helicase like transcription factor LAMA1 Laminin subunit alpha 1 NEUROG1 NEUROG1 Neurogenin 1 NGFR NGFR Nerve growth factor receptor RARB RARB Retinoic acid receptor beta RXRG RXRG Retinoid X receptor gamma RYR2 RYR2 Ryanodine receptor 2 SDC2 SDC2 Syndecan 2 SEPT9 SEPT9 Septin 9 SFRP2 SFRP2 Secreted frizzled-related SOCS3 SOCS3 Suppressor of cytokine signaling 3 SOX17 SOX17 SRY-box 17 THBD THBD Thrombomodulin Transmembrane protein with TMEFF2 TMEFF2 EGF-like and two follista- tin-like domains 2 UCHL1 UCHL1 Ubiquitin C-torminal hydro-lase L1 VIM VIM Vimentin Gene (a) Chromosomal (region) location (a) ADHFE1 8q12.
4] Genes: E6, E6 transforming protein [human papillomavirus 18]; E2, E2 regulatory protein [human papillomavirus]; E7, E7 transforming protein [human papillomavirus]; TP53, tumor protein p53; IFNG, interferon, gamma; TMC6 (previously known as EVER1), transmembrane channel-like 6; TMC8 (previously known as EVER2), transmembrane channel-like 8; DAPK, death-associated protein kinase 1; CDH1, cadherin 1, type 1, E-cadherin (epithelial); RASSF1, Ras association (RalGDS/AF-6) domain family member 1; CDKN2A, cyclin-dependent kinase inhibitor 2A; FHIT, fragile histidine triad; MGMT, O-6-methylguanine-DNA methyltransferase; RARB
, retinoic acid receptor, beta.
85) See KLB (CA), supra note 48 at para 49, aff'd 2003 SCC 51, 2 SCR 403, 230 DLR (4th) 513; BG, supra note 84 at paras 61-66; RARB
, supra note 19.
Furthermore, the assay detected 1 methylated allele in a background of 10 000 unmethylated alleles in the duplicate reactions that amplified GSTP1 and in 1 of 2 replicates of the APC and RARB
amplifications (see Table 10 in the online Data Supplement).
The authors found BRCA1, WT1, and RARB
to be the most frequently methylated tumor suppressor genes, with receiver operating characteristic curve analyses revealing significant diagnostic accuracies in 2 additional validation sets.
We selected 3 methylation markers for a multiplexed PCR assay in a nested (2-round) format, namely, GSTP1, RARB
, and APC.
2003), suggesting the linkage between ALDH6 and RARB
in Figure 2.
120,121 The first analyzed 11 genes in 46 PENs and revealed hypermethylation in the following 8 genes: RASSF1A (75%), P16/INK4A (40%), MGMT (40%), MLH1 (23%), APC (21%), E-cadherin (23%), P73 (17%), and RARB
(25%); no methylation was found in TIMP3, P14, and GST.