After being challenged with 10, 100 and 1000 ng/mL rBla g 7 or medium alone for 48 h and challenged with 1000 ng/mL rBla g 7 (or medium alone) for 20 min, 40 min, and 60 min, the purified DCs were lysed as previously described .
Upregulation of TIM4 Expression on DCs by rBla g 7.
Induction of Maturation and Activation of DCs by rBla g 7.
Induction of Enhanced Expression of IL-13 by rBla g 7.
rBla g 7 Activates NF-[kappa]B Signaling Pathway in DCs.
Induction of Th2 Polarization by rBla g 7-Activated DCs.
Inhibition of rBla g 7-Activated DCs Induced Th2 Polarization by TIM4, CD80, and CD86 Antibodies.
As CD80 and CD86 have been found to mediate the necessary costimulatory signals to evoke Th2 polarization  involved in allergy, CD80 and CD86 upregulation induced by rBla g 7 in DCs may contribute to the subsequent allergic responses.
However, unlike Per a 10 and fungal allergens , rBla g 7 was found to only downregulate the level ofIL-12 mRNA, but not IL-12 secretion from DCs.
Since antibodies against TIM4, CD80, and CD86 blocked rBla g 7-activated-DCs induced IL-13 release from CD4+ T cells, the actions of rBla g 7 on IL13 secretion from CD4+ T cells are likely via TIM4, CD80, and CD86 dependent signaling pathways in DCs.