RBMECRat Brain Microvascular Endothelial Cells
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Migration of RBMEC was detected using a "scratched wound assay." Briefly, RBMEC (3 x [10.sup.5]) was seeded in 12-well plate and grown to confluence.
(a) RBMEC cells treated with GO6976 or vehicle control were cultured in normoxic condition (21% [O.sub.2], 5% C[O.sub.2]) or under hypoxia (1% [O.sub.2], 5% C[O.sub.2], and 94% [N.sub.2]) for 12 or 24 hours.
((c) and (d)) Expression change of HIFia, VEGFa, and P-HDAC4 in RBMEC cells cultured in the model of hypoxia.