The quantity of Wsmax and Wmax performed by the subjects during each minute of the muscle endurance test was not statistically different (range of statistical power 0.12-0.18) before and after RBML (Figure 2A,B).
Pooling the data obtained before and after RBML revealed a significant relationship between body mass and absolute peak torque at angular velocities of 1.57 rad x [s.sup.-1] (r = 0.604; p < 0.001), 3.14 rad x [s.sup.-1] (r = 0.550; p = 0.001) and 4.71 rad x [s.sup.-1] (r = 0.613; p < 0.001).
A significant increase was observed in haemoglobin concentration (p = 0.0008) and haematocrit (p = 0.0008) as a result of RBML (Table 1).
RBML caused an average 6.0 [+ or -] 4.4% decrease in plasma volume.
The concentration of ammonia in the blood plasma of the subjects increased significantly (p < 0.001) as a result of the performance tests before as well as after RBML (Table 1).
There was a significant increase (p < 0.001) in the concentration of lactate in the blood plasma of the subjects induced by the performance tests on both occasions, before and after RBML (Table 1).
However, a pronounced increase of the level of urea (20.4%; p = 0.003) was evident as a result of RBML (Table 1).
The net loss of body water is considered to be the main mechanism through which RBML is achieved within a limited time period (Wilmore, 2000).
The nutritional data of our subjects show that in addition to the overall food energy consumption, the carbohydrate intake was also very low during the RBML period.
The main finding of the present study was a significant reduction in Wtot performed during the 3-min muscle endurance test after RBML in comparison with the value in Test 1.
Thus, our finding extends the current knowledge about the effects of RBML demonstrating that it reduces physical performance capacity even during short periods of high intensity exercise.
A tendency towards lower working capacity after 4.3% RBML was evident in karatekas (Oopik et al., 1998).