RBPTRose Bengal Plate Test
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Through RBPT it was found out that, 118 (12.29% +- 0.0105) samples of goats out of 960 and 91 (19.32% +- 0.289) serum samples of sheep out of 471 were seropositive.
RBPT was performed with commercial Brucella antigen (Crescent Diagnostics, KSA).
The serum samples from both dams showed positive titre of 1:80 (160 IU) in serum agglutination test and rapid agglutination when tested against Rose Bengal Plate Test (RBPT) antigen (Fig.8).
Among the 386 sera screened for Brucella antibodies, 37 (9.6%) were positive for Rose Bengal Plate Test (RBPT).
Therefore, this study was carried out to evaluate diagnostic performance and accuracy of Rose Bengal Plate test (RBPT) and indirect Enzyme-Linked Immunosorbent Assay (i-ELISA) tests for screening and confirmation of bovine brucellosis using Bayesian method.
The results also highlight the usefulness of qPCR as a complementary assay to a combined ELISA and RBPT diagnostic approach in diagnosis of acute brucellosis and the need to establish national and regional reference laboratories with facilities for performing qPCR assays.
All the samples were subjected to RBPT, STAT and I- ELISA, the results of which are presented.
Serum samples of 48 goats, from all the houses of the contacts with possible exposure, were also subjected to RBPT. Health education lectures were organized to create awareness among the high-risk groups (farmers, shepherds, and animal handlers) regarding brucellosis involving the Community Medicine Department, Gram Panchayat, veterinary hospital, and Primary Health Center, Kadoli, Karnataka, India.
Diagnostic Tests: For serodiagnosis, Rose Bengal plate test (RBPT) and serum agglutination test (SAT) were performed following procedures described previously (Aldomy et al., 2009).
The pre-inoculation screening of the lambs for Brucella infection through RBPT revealed absence of antibody, implying that the herd was free from prior infection or sensitization.
From 360 tested samples, 24 (6.6%) were found to be serologically positive by RBPT and the rest of the cows were serologically negative by RBPT (Figure 1).