REP-PCRRepetitive Extragenic Palindromic Sequence Polymerase Chain Reaction (genetics)
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cereus isolated from patients and equipment in the hospital, which we named Tokyo strains, we performed PFGE analysis and rep-PCR fingerprinting.
Then we used the 16S rDNA sequencing methods and rep-PCR methods to identify and characterize these Mycoplasma isolates.
Bradic, "Genomic fingerprinting of Bradyrhizobium japonicum isolates by RAPD and rep-PCR," Microbiological Research, vol.
Almost CRAB strains in this group showed the synergy or additive effect of colistin plus sulbactam, whereas the other REP-PCR groups (B-H) mostly showed indifferent effect of a couple of antimicrobials.
Abbreviations CAAT: Cross agglutinin absorption test D: Discriminatory indices EMJH: Ellinghausen-McCullough-Johnson-Harris ERIC: Enterobacterial repetitive intergenic consensus MAT: Microscopic agglutination test MLST: Multilocus sequencing typing PFGE: Pulsed field gel electrophoresis RAPD: Random amplified polymorphic DNA Rep-PCR: Repetitive element-based PCR UPGMA: Unweighted pair group method with arithmetic mean UV: Ultraviolet.
Abbreviations AFLP-PCR: Amplified-fragment length polymorphism polymerase chain reaction BP: Biological process BWA: Biological warfare agents CC: Cellular component CDC: Centers for Disease Control and Prevention DE: Differentially expressed or differential expression HKGs: Housekeeping genes hPBMCs: Human peripheral blood mononuclear cells MF: Molecular function miRNAs: MicroRNAs NIAID: National Institute of Allergy and Infectious Diseases PPC: Positive PCR controls REP-PCR: Repetitive element polymerase chain reaction RT-PCR: Real-time polymerase chain reaction RTC: Reverse transcription controls.
epidermidis isolates than REP-PCR and RAPD methods (Begovic et al., 2013).
Todas las PCR se realizaron en un volumen final de 25[micro]l que contenian 1X amortiguador de reaccion, 2mM Mg[Cl.sub.2], 0,2mM dNTP's, 2[micro]M iniciadores, 10ng ADN de la muestra bacteriana y con respecto a la Taq Polimerasa, se emplearon 0,625 U para las PCR con iniciadores especificos y 1 U para las ERIC-PCR y REP-PCR.
KEYWORDS: Epidemiology, ESBL producing E.coli, Quinolone resistance, Clonal relationship, rep-PCR.