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Rapid fluorescence focus inhibition technique (RFFIT) is a gold standard technique for the determination of RVNA titers (Bahloul et al., 2002).
Sera were separated, stored at -80degC before shipping these to K-State Rabies Laboratory at Kansas State University, Manhattan, K.S., USA for mRVNA screening by rapid fluorescent focus inhibition technique (RFFIT).
The RFFIT is one of the most widely used substitutes to the Mouse Neutralization Test (MNT) and is a rapid test that requires only 20 hours for completion and is slightly more sensitive than the MNT for detecting virus neutralizing antibodies (VNAs) in postvaccinal sera [35, 36].
The RFFIT assay is also used by FADL to evaluate the titer of veterinarians, animal handlers, and other personnel with potential rabies exposures.
In the United States, ante-mortem rabies testing requires four specimens (serum, CSF, nuchal skin biopsy, and saliva), with serum and CSF tested by IFA and RFFIT. All persons potentially exposed to rabies virus should receive an individual risk assessment to determine if PEP is indicated.
Rabies virus neutralizing antibody (RVNA) titers as determined by Rapid Florescent Focus Inhibition Test (RFFIT) can indicate vaccine efficacy.
Sufficient quantity of serum for RFFIT was available from only 165 (85%) of the sampled animals, which were included in this study.
rabies patients, antibodies to the abundant viral nucleocapsid antigens detected by IFA are registered first, whereas VNA, directed to the outer viral glycoprotein, are only detected later by RFFIT, ifVNA are detected at all.
The threshold for RFFIT positivity chosen in this study was slightly higher than that used in recent bat studies performed in Europe (3) and the Philippines (6).
Serum and CSF samples obtained December 21 contained rabies virus neutralizing antibody titers of 1:50 and 1:36, respectively, by rapid fluorescent focus inhibition test (RFFIT).
At the Centers for Disease Control and Prevention (CDC), the presence of virus-neutralizing antibodies was determined by a modification of the rapid fluorescent focus inhibition test (RFFIT) (18).
If titers are obtained, specimens collected 2-4 weeks after preexposure or postexposure prophylaxis should completely neutralize challenge virus at a 1:25 serum dilution by the rapid fluorescent focus inhibition test (RFFIT).
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