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HEPES-buffered saline for intracellular [Ca.sup.2+] level measurements contained 145 mM NaCl, 10 mM HEPES, 10 mM D-glucose, and 5mM KCl, pH 7.4, supplemented with 0.1% (w/v) BSA, 100 [micro]M RGDS, 200 [micro]M Ca[Cl.sub.2], and 0.1 U/mL apyrase.
For measurements of intracellular [Ca.sup.2+] levels, PRP was prepared as described above and RGDS peptide was added to prevent aggregation .
The news is positive for the shares of RGDs, in our view.
The surface modification of CTNPs with RGDS peptide was confirmed by FTIR as displayed in Figure 3.
Moreover, although fibrinogen is essential for Ni[Cl.sub.2]-induced platelet aggregation, it is not required for Ni[Cl.sub.2]-induced platelet activation, expressed as [p60.sup.src] phosphorylation, as demonstrated by the fact that such response is not inhibited by the incubation with the tetrapeptide RGDS (120 [micro]g/mL), which prevents fibrinogen binding to its receptor, the integrin [Alpha]IIb[Beta]3 (8).
Although Sechin's letter to the President indicates that the appraisal of the RGDs may be finalised soon, its completion may not necessarily mean the consequent deal completion given that serious tensions exist between Rosneftegas and Gazprom and because other concerns have surfaced.
The news is positive for the RGDs' shares as it looks like the deal may be finalised in line with our expectations.
This is the third time that Rosneftegas has conducted an appraisal of the RGDs' value.
The stakes in 72 RGDs have an estimated value of RUB26.6bn, while the market valueof the 0.89% stake in Gazprom (which will pass to Rosneftegas) is RUB40.6bn.
- The first stage of the deal was completed in Jan 2009 when Rosneftegas exchanged its shares for minority stakes in RGDs with Rosimushestvo as part of the privatization process.
Since the process of surface coating by simple protein adsorption does not provide a high level of control over the presentation of cell-binding domains, different strategies have been idealized to covalently immobilize cell-binding motifs (e.g., RGD) on a substrate [86, 87].
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