The staining of RHAMM was graded semiquantitatively and the H-score was calculated using the following equation: H-score= [SIGMA]Pi (i + 1)where i = intensity of staining with a value of 1, 2 or 3 (weak, moderate or strong, respectively) and Pi is the percentage of stained cells for each intensity, varying from 0 to 100.
The most intense immunoreactivity of RHAMM was in the epithelium on D4 (275.
RHAMM stimulates cell migration and locomotion via activation of a signal transduction cascade upon HA binding (2).
We observed that the RHAMM immunoreactivity of uterine epithelial cells and subepithelial cells increased in proestrus compared with estrus and diestrus cycles (Table 1).
It is known that RHAMM is highly expressed in the G2/M phase of the cell cycle, thus, controlling mitosis (14).
Cell biologist Mina Bissell collaborated with Eva Turley, an oncology professor at the University of Western Ontario, to study the role that RHAMM plays in regulating the signalling of fat cells during the repairing of tissue wounds from injuries like skin cuts, heart attacks, and stroke.
The researchers blocked the expression of the RHAMM protein in mice either by deleting its gene or through the introduction of a blocking reagent.
They found that blocking the expression of RHAMM could be used to selectively induce the generation of fat cells to replace those lost in the aging process.
They also observed that blocking RHAMM reduced deposits of unhealthy visceral fat, which suggested that such an approach might also have a beneficial effect on patients with obesity-related diseases, cardiovascular disease or diabetes.
The reactivities of cellular RHAMM sites toward specific anti-RHAMM antibodies and HA were studied comparatively by immunohistochemical experiments in which sponge cells were double-labeled with mouse anti-RHAMM antibodies and with BHA.
The presence of color development at all sites that were blotted with RHAMM peptide and MAF peptide constructs indicated that they were reactive with hyaluronic acid (BHA).
Taken together, the evidence indicates that (a) the postulated HA binding sequence of MAF core peptide actually functions in this role as determined by the behavior of an HA binding mimetic, and (b) this MAF HA binding sequence, in addition to the RHAMM on Microciona cell membranes, may serve as anchorage points for HA molecules.