RHKORandom Homozygous Knock Out
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In this case, the RHKO strategy enabled the researchers to isolate a defective gene in breast cancers that weren't necessarily familial.
But the random-hit, bacteria-style experiment was not possible in animal cells until last May, when Li and Cohen first reported their RHKO method in Cell.
Meanwhile, scientists using the RHKO method may discover other new, important genes for study.