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En el analisis detallado de la secuencia de los genes dependientes del QS (rhlA, rhlB, rhlR, rhlI, etc), Grosso et al.
El papel de la proteina RhlA en la sintesis de ramnolipidos (rhls)y polihidroxialcanoatos (PHAs)en Pseudomonas aeruginosa.
rhlA is required for the production of a novel biosurfactant promoting swarming motility in Pseudomonas aeruginosa: 3-(3-hydroxyalkanoyloxy) alkanoic acids (HAAs), the precursors of rhamnoli pids.
Efecto de RhlA en la produccion de ramnolipidos y polihidroxialcanoatos en Pseudomonas aeruginosa (PAO1) Tesis, Biologo.
The serum was adsorbed with DQA1*02 : 01/DQB1*04 : 01 rHLA cells and the eluted antibody reacted with DQ4, DQ5, and DQ6 antigens which share epitope 2007 (Table 7).
The efficacy of adsorption and elution assays is demonstrated where one serum with DQ specificity, including DQA1*02 : 01, underwent four separate adsorptions and elutions with rHLA DQ cells.
The results showed that high-expression level (0.9-4 [micro]g/mL) of rhLA was found in eight of 45 colonies in the culture medium.
The milk (5 [micro]L) from goats BC186 and BC228 with four and two copies of transgene was collected and analyzed using Western blot and ELISA to determine the presence of rhLA, which has a very similar migration compared with human milk-derived LA (Figure 3).
Goats were lactated with hormone and milked from day 7 through day 65 of lactation, and the milk was assayed for rhLA. Recombinant protein in the milk was present at concentrations between 0.1 and 0.9 mg/mL.
Relative expression using qRT-PCR of biofilm forming encoding genes: cupA1 and lasI and virulence factor encoding genes: rhlA, lasB, plcH, and aprA showed that the transcription levels of the mRNA transcripts among biofilm forming populations differed from that in their planktonic counterparts for a number of genes, especially the hemolytic phospholipase C encoding gene plcH, which was up-regulated in the biofilm cultures of all selected isolates (Table 4, Figure 2).
Isolate Serial Genotype Biofilm Forming Encoding Name # Genes Real Time RT-PCR: Gene Expression Level (Quantitative) Planktonic Biofilm Cultures Cultures cupA1 lasI cupA1 lasI Pa PA14 1 1 1 1 Pa N5 23 0.93 0.4 0.94 0.1 Pa N44 1 1.31 0.1 1.27 0.2 Pa N58 10 1.1 0.1 1.4 0.01 Pa N59 1 0.5 2.0 0.8 0.03 Pa N68 5 0.4 0.5 0.3 0.2 Pa CA1 0.6 1.3 1.2 0.1 Isolate Virulence Factor Encoding Genes Name Real Time RT-PCR: Gene Expression Level (Quantitative) Planktonic Cultures Biofilm Cultures rhlA lasB plcH aprA rhlA lasB plcH aprA Pa 1 1 1 1 1 1 1 1 Pa 0.6 0.2 1.004 0.3 0.2 0.02 3.5 0.4 Pa 0.4 0.2 0.2 0.7 0.1 0.01 2.4 0.1 Pa 0.3 0.05 0.6 0.5 0.1 0.04 0.7 2.03 Pa 2.5 2.4 0.2 2.3 1.7 0.3 3.2 3.1 Pa 1.9 I.I 1.2 3.7 3.2 0.4 1.5 5.01 Pa 2.4 2.5 0.6 4.9 5.3 0.3 3.7 2.3
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- RHM Kwok