The integrated approach of the SWE and RKC
construct will continue to drive readiness and improve processes for optimal Fleet support.
1994), sequences representing RKC 18S and COI genes were aligned with other sequences from decapods, including anomurans, available from GenBank and provided by colleagues.
The level of variation in 18S between RKC and blue king crab sequences was insufficient for species-specific primer and probe design.
Because inhibition was not detected during RT-PCR using DNA derived from adult RKC muscle tissue, the IPC assay was not included regularly in reactions targeting DNA from adult tissues.
A plasmid DNA control encoding the target COI sequence was created by ligating a Paca3F/Paca3R-generated RKC amplicon into a vector using the TOPO TA Cloning Kit for Sequencing (Invitrogen) according to the manufacturer's instructions.
A silicon-intensified tube (SIT) camera, which uses ambient light, was attached to the center of the trawl to view RKC interaction with the footrope.
For each tow the total catch of all species from each net was first weighed before all RKC were removed from the catch, weighed, coded by sex, and measured to the nearest millimeter.
footrope, a video camera was mounted on the trawl to observe RKC interaction with the center of the trawl footrope.
The two-parameter model (model 1) of capture probability was selected over the three-parameter model (model 2) because it had a lower AIC value for both male and female RKC (Table 2).
After juvenile RKCs had molted to the C3 size class, 36 crabs were transferred to a second type of culture system that consisted of individual culture cells made from stiff mesh tubing cut to 17.5 cm high (for further details, see Stoner et al.
Experiment 2: Comparison of Lipid Classes and Fatty Acids of Hatchery and Wild Juvenile RKCs
Crabs were fed commercially available feeds including Cyclopeeze (Argent Chemical Laboratories, WA), Otohime B1 and B2 fish feed (Reed Mericulture, CA), frozen and enriched Artemia nauplii, and Zeigler (Zeigler Bros., Inc., PA) shrimp feed, which have been used to culture juvenile RKCs successfully (Daly et al.