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Test of Labelled rMBL
. The time-resolved immunofluorometric assay (TRIFMA) for quantification of MBL has previously been described in detail .
Test of Function of the AF680 Labelled rMBL
. FluoroNunc MaxiSorp microtiter plates (Thermo Fisher Scientific, MA, USA) were coated with 10 [micro]g/mL mannan in carbonate coating buffer (15 mM [Na.sub.2]C[O.sub.3], 35 mM, NaHC[O.sub.3], and 15 mM Na[N.sub.3], pH 9.6).
The scale of introduction was intentionally modest because RMBL
was concerned about the potential for nonnative spread.
Director Ian Billick comments on the measures necessary to protect the lab's irreplaceable documents.
He made his first pilgrimage to RMBL
in 1977--to study acid rain in local lakes--and he's been back every summer since, occupying the same little cabin since 1980.
We studied the montane perennial herb, Ipomopsis aggregata subspecies aggregata (Polemoniaceae; Grant and Wilken 1986), in meadows near the Rocky Mountain Biological Laboratory (RMBL
), Gothic, Gunnison County, Colorado, USA Ipomopsis aggregata is self-incompatible and does not reproduce vegetatively (Waser and Price 1983).
Sacco for field, financial, and administrative assistance at the RMBL
We worked in a subalpine meadow at the Rocky Mountain Biological Laboratory (RMBL
) in west-central Colorado (38 [degrees] 53[minutes] N, 107 [degrees] 02[minutes] W, elevation 2920 m).
- This study took place at the Rocky Mountain Biological Lab (RMBL
), between 13 July and 10 August of 1993.
(Diptera: Anthomyiidae) seed predators were heavy, and so in 1987-1989 I instead potted the plants and moved them to a portable greenhouse (Hansen WeatherPort) at the RMBL
. Crosses were performed using toothpicks to transfer pollen from freshly deshisced anthers to receptive stigmas.
) fell 7.5% to close at $4.30 following downbeat Q2 earnings.