RNA-PCRReversed Transcription and Subsequent Polymerase Chain Reaction
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References in periodicals archive ?
Optimisation of DNase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR. Biotechniques 1996;20:1012-20.
Oligo(dT)-immobilized PP microplates (GenePlate-PP, AGCT), Yoyo-1 (Molecular Probes), reagents for PCR [Taq polymerase and EZ rTth RNA-PCR kit (Perkin-E1mer)], the K562 cell line (American Type Culture Collection), a 100-bp DNA ladder, phosphate-buffered saline, vanadyl ribonucleoside complex, rabbit globin mRNA, cell culture medium and appropriate antibiotics, buffer-saturated phenol, biotin-dATP (Life Technologies), fetal bovine serum (HyClone), biotin-dUTP, primers for glyceraldehyde-3-phosphate dehydrogenase (G3PDH; Clontech), AttoPhos (JBL Scientific), Genius hybridization buffer, LumiPhos (Boehringer Mannheim), and supported nitrocellulose membranes (Optitran, Schleicher & Schuell) were purchased from the designated suppliers.