RF

(redirected from RNase-Free)
AcronymDefinition
RFRadio Frequency
RFRight Front
RFReference
RFRight Field (baseball)
RFRising Force (Yngwie J. Malmsteen album)
RFRegions Financial Corporation (stock symbol)
RFRheumatoid Factor
RFRange Factor (arcane baseball statistic)
RFRisk Factor
RFRange Finder (camera)
RFRockford Fosgate (car audio)
RFRed Faction (game)
RFRenal Failure
RFRiksidrottsförbundet (Swedish)
RFRight Foot (dance)
RFRheumatic Fever
RFRascal Flatts (country music artist)
RFRelease Factor
RFReform Party (founded by Ross Perot in 1995)
RFReduction Factor
RFRépublique Française (French Republic)
RFRegister File
RFRelative Frequency
RFRockefeller Foundation
RFRiver Forest (suburb of Chicago, IL)
RFReaction Force
RFRaised Face (flange)
RFReceptive Field
RFRevolving Fund
RFResponse Factor
RFRim Fire (ammunition)
RFReverend Father
RFReserve Force
RFRadiative Forcing
RFRNase-Free
RFresponse force (US DoD)
RFReplication Factor
RFRighteous Fury (gaming, World of Warcraft)
RFRear Floor (automobile manufacturing)
RFRecovery Factor (oil and gas)
RFRegional Forces (Vietnam)
RFRepresentative Fraction
RFRipper Free (DVD backup software without ripping decoders)
RFResume Flag
RFRhodesian Front (political party)
RfRetardation Factor (chromatography)
RFRespirable Fraction
RFRepair Factor
RFRear Focusing (lens technology)
RFRainforest Foundation (UK)
RFRadiated Frequency
RFRegistered Forester
RFReconnaissance Fighter
RFRipping Friends
RFRadio Fantasy
RFReconocimiento de Formas (Spanish)
RFRossiyskaya Federatsiya (Russian Federation)
RFReplacement Factor
RFReaction Function (economics)
RFReceptor Factor(s)
RFRelative Body Fat
RFReadiness Field
RFReaction Furnace
RFReal Feel Temperature (index created by AccuWeather)
RFReflexology Forum
RFResistance to Frequency
RFRatio of Fronts (component)
RFReprographic Facility
RFRalph Furley
RFReduced Fratricide
RFRelentless Family (gaming clan)
RFRandomflame (gaming community)
RFRefrigerant Ton
RFUS Revenue Playing Cards (Scott Catalogue prefix; philately)
RFRotary Foundation
RFRadius to Fix
RFRolling Forecast (finance & marketing)
RFRutherfordium (element 104)
RFRoyal Fusiliers (WWI British Army regiment)
RFRoyal Family (UK)
RFRight-Arm Fast (cricket; also seen as RAF)
RFRussian Federation
RFRoger Federer (tennis player)
RFRoskilde Festival (Danish music festival)
RFRural Friends
RFRumble Fighter (online gaming)
References in periodicals archive ?
In order to synthesize cDNA, 2 [micro]g of RNA, 1 [micro]L of oligo-dT (Invitrogen, Germany), and 1 [micro]L of RNase-free water were added, the mixture was denatured at 80[degrees]C for 3 min, and then the cDNA was synthesized using 4 [micro]L of 5x RT buffer, 5 [micro]L of 2 mM deoxynucleotide (dNTP), 0.
The precipitate was then dissolved with a proper amount of RNase-free H2O, providing purified RNA.
5 u l of Taq Polymerase (5U/ul), 5 u l of 10X Taq buffer, 1 u l of each type specific primers P[1], P[5] and P[11] and an upstream primer Con2 and RNase-free water to final volume of 50 l, and subjected to nested multiplex PCR amplification by following the PCR cycles as reported by Falcone et al.
The real time quantitative PCR (RT-qPCR) was performed in 20 ul reactions containing 10 ul 2 x SYBR Premix Ex TaqII (Tli RNase Plus) (Takara), 1 ul of each primer, 1 ul of 1: 10 diluted cDNA templates and 7 ul RNase-free H2O.
After the residual liquid was removed, the pellet was resuspended in 30 [micro]L of ultrapure RNAse-free water.
The pellet was air dried, resuspended in 20 [micro]L RNase-free water, and stored at -80 [degrees]C.
Total RNA extracted from cells using TRIzol was treated with RNase-free DNase I, and then reverse transcribed using PrimeScript RT reagent kit.
Posteriormente se realizo la destruccion del ADN, mediante el uso de RNase-Free DNase Set (Quiagen ).
Embryos were transferred to RNase-free microcentrifuge tubes, and RNA was isolated according to a protocol supplied by the manufacturer.
Basic precautions to inhibit RNA degradation by RNase include: performing extractions at 4 [degrees] C, storing mRNA in the appropriate solvent at -80 [degrees] C, and sterilizing all glass and plasticware to be RNase-free.
from freshly dissected mudpuppy nasal tissue; it was treated with RQ1 RNase-free DNase (Promega), and converted to first strand cDNA with Superscript II Reverse Transcriprase (Gibco BRL).