(redirected from RNase-Free)
RFRadio Frequency
RFRight Front
RFRight Field (baseball)
RFRising Force (Yngwie J. Malmsteen album)
RFRussian Federation
RFRegions Financial Corporation (stock symbol)
RFRheumatoid Factor
RFRoger Federer (tennis player)
RFRange Factor (arcane baseball statistic)
RFRoyal Family (UK)
RFRisk Factor
RFRed Flag
RFRange Finder (camera)
RFRockford Fosgate (car audio)
RFRate of Fall (US Air Force)
RFRed Faction (game)
RFRenal Failure
RFRoyal Flush (cards)
RFRiksidrottsförbundet (Swedish)
RFRutherfordium (element 104)
RFRight Foot (dance)
RFRoskilde Festival (Danish music festival)
RFRetractable Fastback (Mazda MX-5 Miata)
RFResponse Force (US DoD)
RFRumble Fighter (online gaming)
RFRight Forward
RFRheumatic Fever
RFRascal Flatts (country music artist)
RFRelease Factor
RFRoyal Fusiliers (WWI British Army regiment)
RFReduction Factor
RFReform Party (founded by Ross Perot in 1995)
RFRadius to Fix
RFRépublique Française (French Republic)
RFRegister File
RFRelative Frequency
RFRotary Foundation
RFRockefeller Foundation
RFRiver Forest (suburb of Chicago, IL)
RFReaction Force
RFRaised Face (flange)
RFReceptive Field
RFRevolving Fund
RFResponse Factor
RFRolling Forecast (finance & marketing)
RFRim Fire (ammunition)
RFRhodium Finish
RFReverend Father
RFReserve Force
RFRadiative Forcing
RFReplication Factor
RFRighteous Fury (gaming, World of Warcraft)
RFRear Floor (automobile manufacturing)
RFRight-Arm Fast (cricket; also seen as RAF)
RFRecovery Factor (oil and gas)
RFRegional Forces (Vietnam)
RFRepresentative Fraction
RFRipper Free (DVD backup software without ripping decoders)
RFResume Flag
RFRhodesian Front (political party)
RFRetardation Factor (chromatography)
RFRural Friends
RFRespirable Fraction
RFRepair Factor
RFRear Focusing (lens technology)
RFRainforest Foundation (UK)
RFRadiated Frequency
RFRegistered Forester
RFReconnaissance Fighter
RFRipping Friends
RFRadio Fantasy
RFReconocimiento de Formas (Spanish)
RFRossiyskaya Federatsiya (Russian Federation)
RFReplacement Factor
RFReaction Function (economics)
RFReceptor Factor(s)
RFRelative Body Fat
RFReadiness Field
RFReaction Furnace
RFReal Feel Temperature (index created by AccuWeather)
RFReflexology Forum
RFResistance to Frequency
RFRatio of Fronts (component)
RFReprographic Facility
RFRalph Furley
RFReduced Fratricide
RFRelentless Family (gaming clan)
RFRandomflame (gaming community)
RFRefrigerant Ton
RFUS Revenue Playing Cards (Scott Catalogue prefix; philately)
References in periodicals archive ?
Each reaction mixture contained 10 uL SYBRA(r) Premix Ex TapTM II, 1 uL of each primer (10 uM), 0.5 uL of ROX Reference Dye II, 1 uL of cDNA and 6.5 uL of RNase-free water in a final volume of 20 uL.
To synthesize cDNA, 1 [micro]g of RNA and 1 [micro]L each of oligodT (Invitrogen, USA) and RNase-free water were added.
Each 20 [micro]L reaction solution contained 10 [micro]L 2xmiRNA Reaction Buffer Mix, 2 [micro]L 0.1% BSA, 2 [micro]L miRNA PrimeScript RT Enzyme Mix, 1 [micro]L total RNA, and 5 [micro]L RNase-Free [H.sub.2]O; qRT-PCR was carried out for Initial activation of 95[degrees] C for 10 s, followed by 40 cycles of 95[degrees]C for 5 s, 60[degrees]C for 20 s.
The precipitate was then dissolved with a proper amount of RNase-free H2O, providing purified RNA.
Briefly, 5 u l of undiluted first RT-PCR product was added to a reaction mixture consisting of 3 u l of 25mM MgCl2, 2 u l of 10mM dNTPs, 0.5 u l of Taq Polymerase (5U/ul), 5 u l of 10X Taq buffer, 1 u l of each type specific primers P[1], P[5] and P[11] and an upstream primer Con2 and RNase-free water to final volume of 50 l, and subjected to nested multiplex PCR amplification by following the PCR cycles as reported by Falcone et al.
Subsequently, a nested PCR was carried out by using the following: 3 [micro]l of RT-PCRproduct, master-mix: 14 [micro] l, forward and reverse primers: 1 [micro]l each, and RNase-free water: 6 [micro]l to make a final volume of 25 [micro]l.
Reaction was performed in RNase-free water and incubated for 10 min at 37[degrees]C with shaker at low speed (300 rpm).
The following materials were purchased from the manufacturers indicated: RNeasy Kit and RNase-Free DNase from Qiagen (Hilden, Germany); High-Capacity cDNA Reverse Transcription Kit and Power SYBR Green PCR Master Mix from Applied Biosystems (Foster City, CA); GenElute PCR clean-up kit, 3-3'-diaminobenzidine, heparinase I and III blend from Flavobacterium heparinum, and heparan sulfate from Sigma-Aldrich (St.
The cDNA was synthesized in a 0.2 mL RNAse-free microtube, starting with 5.0 [micro]L of viral RNA.
The real-time PCR reaction system was a 10 [micro]L reaction mixture containing 0.5 [micro]L cDNA, 4.1 [micro]L RNase-free water, 5 [micro]L 2x SYBR Green Master Mix, and 1.8 [micro]L of each primer (10 mM).
Materials required were the following: buffer RAV1 2x 35 mL, buffer Raw 2x 30 mL, buffer RAV3 (concentrated) 2x 125 mL, buffer RE 2x 5 mL, carrier DNA lyophilized 2x 1mg, Ribo Virus columns 100, collection tubes (2) 300, ethanol 96-100%, biological cabinet, microcentrifuge tubes, sterile RNAse-free pipette tips with aerosol barrier, disposable gloves (powder-less), microcentrifuge (with rotor for 2mL tubes), and real time qPCR machine.
After the microcentrifuge tube was dried, the RNA pellet was resuspended in 20 [micro]L of ultrapure RNase-free water.