RP-HPLCReversed Phase-High Performance Liquid Chromatographic Method
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The fraction V with the highest ACE inhibitory activity was selected for further separation by RP-HPLC.
In RP-HPLC chromatogram, a small peak was also observed at a retention time of 40-45 min., which showed the mix type of sequence (data not shown).
A simple, rapid, sensitive, selective and robust method was developed and validated for quantification of piroxicam and 5-hydroxypiroxicam in spiked human plasma, using isocratic RP-HPLC connected to UV detector.
Proteolysis in freeze dried soluble fraction (pH 4.6) of LFMCs were determined on an RP-HPLC system (Shimadzu, Japan) consisted of C18 column (Shim-pack CLC-ODS, 250 cm x 4.6 mm, 5 um) equipped with pump (LC-10AT, Shimadzu, Japan), an auto-sampler and UV detector (SPD-10AV, Shimadzu, Japan).
The isocratic RP-HPLC method was carried out using Thermo Scientific[TM] Dionex-UltiMate[TM] 3000 HPLC system equipped with solvent reservoirs, LPG3400SD pump, WPS-3000 autosampler injector, TCC-3000 column oven, and DAD-3000 ultraviolet-visible (UV-Vis) diode array detector module operated at four wavelengths per analysis.
Then, lyophilized PS[O.sub.(G25)]3 was purified by RP-HPLC and determined the sequence of Ser-Gly-Pro-Val-Gly-Leu-Trp, which was named as PSO.
Acetylation reaction resulted in a mixture of mono- and multiple acetylated derivatives of [Fe]FSC due to three identical primary amines but the desired products were easily accessible via preparative RP-HPLC purification.
Patil, "Development and validation of a novel RP-HPLC method for simultaneous estimation of alogliptin benzoate and pioglitazone HCL in pharmaceutical dosage form," International Journal of Chemical and Pharmaceutical Analysis, vol.
For this aim, acetic acid-ammonium acetate buffer (pH 5-7.5) has been recently used as a modifier for the purification and separation of many azo dyes by RP-HPLC [19,20,23-25].