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1b) fractionate was checked by using RPHPLC and it is found to be 80.23 [+ or -] 0.25%.
Ghode et al., "An rphplc method for simultaneous estimation of Ceftriaxone sodium and sulbactam sodium in parenteral dosage form," International Journal of Pharmacy and Pharmaceutical Sciences, vol.
Determination of fipronil residue in soil and water in the rice fields in north of Iran by RPHPLC method.
After reaction, the remainder of the A-chain was assembled and the peptide was isolated and purified via RPHPLC. Oxidation of the CysA10-CysA15 pair was performed using 2 equivalents of elemental iodine as described above.
For the RPHPLC analysis, samples filtered using a 0.4[micro] filter paper was used (C18 reverse phase column, Solvents: Acetonitrile: Water = 65:35; 0.1% TFA, flow rate 1 ml/min, Sample injected = 20 [micro]l, Detector: UV-Vis., 210 nm).
The alkylated protein was desalted by RPHPLC on a [C.sub.18] column (Syininetry C18; column, bead, and pore sizes are the same as above) by elution with a 30-min linear acetonitrile gradient (0%-80%) in 1 mL/L TFA.
Robutti G, Borras JL, Ferrer FS, Bietz ME (2000) Grouping and Identification of Argentine Maize Races by Chemometric Analysis of Zein RPHPLC Data.
Therefore, a third chromatographic step was performed, using RPHPLC, resulting in the separation of a major sharp peak from few minor contaminants (Fig.
Often they cannot be easily assayed directly by RPHPLC (due to lack of retention) or GC (due to thermal degradation).
Amin, "RPHPLC method using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate incorporated with normalization technique in principal component analysis to differentiate the bovine, porcine and fish gelatins," Food Chemistry, vol.
Patil, "Sensitive and selective method for the analysis of menthol from pharmaceutical products by RPHPLC with refractive index detector," Journal of Pharmacy and Bioallied Sciences, vol.
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