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Gene Sequences CXCL8 Fwd: 5'-GACTCAGGTTTGCCCTGAGGGGATG-3' Rev: 5'-GCTTGTGTGCTCTGCTGTCTCTGAA-3' IL6 Fwd: 5'-CCAGCCATCCTCCCCCATTTTCATT-3' Rev: 5'-CAGGCTGAAACCAGACCCTTGCACA-3' TNF Fwd: 5'-TGAATGATTCTTTCCCCGCCCTCCT-3' Rev: 5'-CACGTCCCGGATCATGCTTTCAGTG-3' HBB Fwd: 5'-AAGCCAGTGCCAGAAGAGCCAAGGA-3' Rev: 5'-CCCACAGGGCAGTAACGGCAGACTT-3' GAPDH Fwd: 5'-ACCTCCCATCGGGCCAATCTCAGTC-3' Rev: 5 '-GGCTGACTGTCGAACAGGAGGAGCA-3' RPS6 Fwd: 5'-TGCAAAGTGCCTGGGACAGAAGTGG-3' Rev: 5'-CGCAGGTCACATAGGCGCTTTCAGT-3' Table 3: Sequences of ChIP-PCR primers.
(14.) Kim, S.H., Kwon, S.I., Saha, D., et al., Resistance to the Pseudomonas syringae effector HopAl is governed by the TIR-NBS-LRR protein RPS6 and is enhanced by mutations in SRFR1.
Casein kinase 1 (CK1)-dependent phosphorylation of rpS6 promotes its association with the mRNA capbinding complex in vitro and protein phosphatase 1 (PP1) antagonizes rpS6 C terminus phosphorylation and cap binding in intact cells (Hutchinson et al., 2011; Li et al., 2012).
In other experiments, they crossed the transgenic Arabidopsis lines with lines in which the RPS6 protein gene was silenced.
Due to unequal variance, data for rpS6 and 4EBP1 were transformed to square roots prior to statistical analysis.
(23) To confirm the correlation between Notch1 activation and expression of mTOR pathways components, we examined expression of Notch1, 4E-BP1, and rpS6 expression in 5 cases of PEL and 21 cases of plasma cell myeloma (Table 2).
The location of the Rps loci on molecular linkage groups is as follows: Rps1 and Rps7 are located on molecular linkage group (MLG) N (Weng et al., 2001), Rps2 on MLG J (Polzin et al., 1994), Rps3 on MLG F (Diers et al., 1992, Demirbas et al., 2001), and Rps4 and Rps6 on MLG G (Demirbas et al., 2001; Sandhu et al., 2004).
Genes such as RPL11, RPL23, RPS6, and MRPS21 were enriched on this pathway.
Ribosomal protein S6 (rpS6) is a well-characterized downstream molecule that is regulated by p70S6K, and for at least 10 yrs after the discovery of S6K, rpS6 was believed to be its only substrate.
Although not observed after Na/exposure, multiple 40S ribosomal protein transcripts were significantly reduced by PTU and PB treatment, including S4 (Rps4), S6 (Rps6), and S8 (Rps8) proteins (Table 6).
sojae have been placed on MLGs, A2 (Rps8), G (Rps4, Rps6, and possibly Rps5), J (Rps2), and N (Rps1 and Rps7) (Diers et al., 1992; Lohnes and Schmitthenner, 1997; Demirbas et al., 2001; Burnham et al., 2003).
To date, 13 resistance (Rps) alleles at seven loci have been described: Rps1 (Bernard et al., 1957), Rps2 (Kilen et al., 1974), Rps3 (Mueller et al., 1978), Rps4 (Athow et al., 1980), Rps5 (Buzzell and Anderson, 1981), Rps6 (Athow and Laviolette, 1982), and Rps7 (Anderson and Buzzell, 1992).
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- RPS Migration Facility