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RQ-PCR was performed on a 7500thermal cycler (ABI) using the TaqMan microRNA Assay (ABI) with the following program: 50[degrees]C for 2 min and 95[degrees]C for 10 min, followed by 40 cycles of 95[degrees]C for 15 s and 60[degrees]C for 1 min.
The performance of these methods are compared with the existing method RQ-PCR. The performance show that NNLD provides higher efficiency with minimum number of training records.
Six of the CML cases had known BCR-ABL fusion results (using fresh peripheral blood) at diagnosis made by RQ-PCR outside Ghana.
The mutation type of each sample was identified using Table 2, which indicates the [DELTA]CT profile obtained with each RQ-PCR primer depending on the mutation type.
due to marrow fibrosis), analysable metaphases may also be obtained from blood leukocytes Real-time RQ-PCR on PB to establish: * A baseline level * The transcript type (1-2% have atypical BCR-ABL fusion products that are undetectable by standard RQ-PCR) FBC = full blood count; LDH = lactate dehydrogenase; BM = bone marrow; FISH = fluorescence in situ hybridisation RQ-PCR = real-time quantitative polymerase chain reaction; PB = peripheral blood.
Once the genetic fingerprint of a leukaemia cell has been identified, the level of residual cells in the bone marrow can be quickly and accurately tested with the RQ-PCR technology, which uses a fluorescent signal to identify the gene sequence.
As IGH and IGK-Kde rearrangements are highly stable PCR targets with relatively large junctional regions, we have applied in this study these rearrangements for MRD monitoring in B-ALL cases by RQ-PCR.
A broad validation study demonstrated that the 4-level ARQ IS Calibrator Panels are reproducibly manufactured and compatible with the accurate alignment of local RQ-PCR methods to the IS through derivation of laboratory-specific analytic correction parameters (CPs).
The following FAMLF-CS-specific primers were used to amplify a 147-bp product by RQ-PCR: forward primer: 5'-ACCGTTTTGAAATTAGATCC-3' (exons 1/2, nt position: 191-210, GenBank accession no.
Analysis of minimal residual disease in childhood acute lymphoblastic leukemia: comparison between RQ-PCR analysis of Ig/TcR gene rearrangements and multicolor flow cytometric immunophenotyping.
Plasma supernatants were carefully removed, and DNA was isolated by use of a QIAamp DNA Blood Midi Kit (Qiagen) and subjected to real-time quantitative PCR (RQ-PCR) for [beta]-globin (9) on a 7900 Sequence Detector (Applied Biosystems).
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