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Rapid resolution liquid chromatography (RRLC) analysis and studies on the stability of Shuang-Huang-Lian preparations.
and Eleutherococcus senticosus Maxim, using rapid resolution liquid chromatography (RRLC).
RRLC is efficient in separating chemical compounds in a mixture, and MS provides abundant information for structural elucidation of the compounds when tandem mass spectrometry is applied .
In addition, the possibility of detecting the target peptides in these samples with rapid resolution liquid chromatography (RRLC) separation, using an electrospray ionization- (ESI-) ion trap (IT) MS, was tested.
The optimized RRLC elution conditions were as follows: 0.0-25.0 min, 5%-20% B; 25.0-40.0 min, 20-50% B; 40.0-41.0 min, 50-99% B; 40.0-45.0 min, 99% B; 45.045.1 min, 99.0-5.0% B; 45.1-55.0 min, 5.0%.
All extracts were dried under vacuum and stored at -80[degrees]C, which was used for rapid resolution liquid chromatography (RRLC) analysis and biological determination.
Hou et al., "Rapid resolution liquid chromatography (RRLC) analysis and studies on the stability of Shuang-Huang-Lian preparations," Journal of Pharmaceutical and Biomedical Analysis, vol.
2012), as shown by the results of chromatograph, IR, UPLC/HPLC-MS, RRLC (Rapid Resolution Liquid Chromatography) (Zhang et al.
Quality control and discrimination of Angelicae dahurica Radix by RRLC fingerprints combined with chemometrics method.
The supernatant was centrifuged at 16,000 x g at 4 [degrees]C for 10 min before the rapid resolution liquid chromatography (RRLC) analysis.
Samples were prepared in an aqueous solution at a concentration of 1000 ppm; they were then filtered and injected into the HPLC system, an Agilent 1200-series RRLC system (Agilent Technologies, Palo Alto, CA, USA) that was equipped with a binary pump and DAD.
The aqueous extract was stirred in a vortex for 10 min until diluted, filtered with single-use filter unit and directly injected into the HPLC system RRLC 1200 HPLC (Agilent Technologies, Palo Alto, CA) equipped with a binary pump and a Zorbax Eclipse Plus C18 4.6 x 150 mm, 1.8 [micro]m column.
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- RRM-containing coactivator activator/modulator