'The xCELLigence RTCA
eSight will revolutionize cell analysis in life science research,' said Todd Christian, Agilent vice president, and general manager of the company's Cell Analysis Division.
"The xCELLigence RTCA
eSight represents the frontier of cell analysis technology by providing unparalleled information with deep biological insights into cellular function," said Xiaobo Wang, Ph.D., who joined Agilent from ACEA, as general manager of the Flow Cytometry and Real-Time Cell Analysis Business.
Proliferation/viability assays performed on ONCO-DG-1 cell line, was determined in a dose dependent manner by using xCelligence RTCA
The xCEL-Ligence system has got four main units: RTCA
analyzer, the RTCA
SP station, the RTCA
computer with integrated software and disposable E-plate 96 (Figure 2).
The xCELLigence RTCA
instruments use noninvasive biosensors to monitor and quantify cell proliferation, cell health, morphology changes, and attachment quality in a label-free, real-time manner.
The cytotoxic effect of [V.sub.2][O.sub.5] was monitored using an xCELLigence RTCA
as described by manufacturer's instructions (Roche Applied Science and ACEA Biosciences) with slight modifications.
was performed to evaluate the proliferation and viability of ESCC cells following continuous treatment with PAR4-AP.
Real-Time Cell Assay (RTCA
) and Morphological Studies.
instruments utilize gold biosensors embedded in the bottom of microtiter wells to non-invasively monitor the status of adherent cells using the principle of cellular impedance.
We used 3 x [10.sup.6] cells per dish for culture and 5 x [10.sup.4] cells per one E-Plate in real-time cell analyzer (RTCA
FaDu cells (5000 cells/well) were seeded onto 16-well E-plates, integrated with gold microelectrode arrays, and incubated in realtime cell analysis (RTCA
) was carried out with the xCELLigence System (Roche, Mannheim, Germany).