16) (2003) also reported that there were NO-GABA interactions in the paraventricular nucleus neurons that project to the RVLM and the NTS.
Previous studies have demonstrated that the Kolliker-Fuse complex is an important area in relaying the cardiovascular effects of the CnF to the RVLM.
Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP.
A hole in skull surface was drilled for lateral ventricle injections, or the dorsal surface of the medulla was surgically exposed for RVLM microinjections.
The level of ROS in the RVLM was evaluated by the oxidative fluorescence dye dihydroethidium (DHE), as described previously .
1]R and ACE2-Ang-1-7-MasR in SHR; and (3) ExT significantly reduced the level of ROS in the RVLM of SHR.
Because the RVLM is a key region involved in control of resting blood pressure and sympathetic outflow, it was further subjected for detecting the protein levels of ACE, AT: R, ACE2, and MasR in response to ExT, which is helpful for determining the possible mechanism responsible for the effect of ExT on central cardiovascular regulation.
In view of the importance of the NTS and RVLM in regulating cardiovascular function [49,50], they are subjected to further experiments.
We first confirm that the decrease in BP evoked by injection of the SOD mimic tempol into the RVLM is attenuated by ExT in SHR, suggesting that ExT effectively reduced the ROS-mediated cardiovascular function in SHR.
Here we provide new insight into the signaling mechanism by which ExT is capable of improving the balance between Ang II and Ang 1-7 in the RVLM of hypertensive rats.
Caption: Figure 2: Representative western blot (a, c) and densitometric analysis (b, d) of ACE and ATjR in the RVLM and NTS of sedentary or exercised WKY and SHR groups.
Caption: Figure 3: Representative western blot (a, c) and densitometric analysis (b, d) of ACE2 and MasR in the RVLM and NTS of sedentary or exercised WKY and SHR groups.