Audry et al., "Multicenter comparative study of a new ELISA, PLATELIAU RABIES II, for the detection and titration of anti-rabies glycoprotein antibodies and comparison with the rapid fluorescent focus inhibition test
(RFFIT) on human samples from vaccinated and nonvaccinated people," Vaccine, vol.
In each study group, blood samples were obtained from 10 randomly selected patients on days 14, 90, and 360; all serum was frozen and analyzed concurrently in 1989 for rabies neutralizing antibody by the rapid fluorescent focus inhibition test
The presence of virus-neutralizing antibodies was determined by an adaptation of the rapid fluorescent focus inhibition test
(RFFIT), as described (7).
Reactivity of serum samples from bats against four lyssaviruses with rapid fluorescent focus inhibition test
, Cambodia, September 2000-May 2001 Frugivorous bats (n = 32) Virus (a) Negative Positive RABV 30 2 EBLV-1 29 3 ABLY 27 5 LBV 28 4 Total (b) 22 10 Insectivorous bats (n = 114) Virus (a) Negative Positive RABV 110 4 EBLV-1 104 10 ABLY 107 7 LBV 111 3 Total (b) 94 20 All bats (N = 146) Virus (a) Negative Positive RABV 140 6 EBLV-1 133 13 ABLY 134 12 LBV 139 7 Total (b) 116 30 (a) RABV, rabies virus; EBLV-1, European bat lyssavirus-1; ABLV, Australian bat lyssavirus; LBV, Lagos bat virus.
At the Centers for Disease Control and Prevention (CDC), the presence of virus-neutralizing antibodies was determined by a modification of the rapid fluorescent focus inhibition test
All were antibody-negative ([is less than] 0.5 IU/ml) by rabies rapid fluorescent focus inhibition test
when quarantined and remained so during the observation period.