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RQ-PCRReal-Time Quantitative Polymerase Chain Reaction
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Real-time quantitative polymerase chain reaction has been widely performed using a single reference gene, frequently not validated or using genes previously validated under different conditions.
During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus.
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