RTPCR

(redirected from Reverse Transcriptase Polymerase Chain Reaction)
Also found in: Medical.
AcronymDefinition
RTPCRReverse Transcriptase Polymerase Chain Reaction
RTPCRReal Time Polymerase Chain Reaction
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References in periodicals archive ?
Reverse transcriptase polymerase chain reaction was positive in 17.6% of the cases and was negative in 82.4% of the cases (that is 9 out of the 51 cases were positive for RTPCR).
Sensitive detection of occult breast cancer by the reverse transcriptase polymerase chain reaction. J Clin Oncol 1994;12: 475-82.
Rapid testing for influenza antigens in a small number of patients was less sensitive than reverse transcriptase polymerase chain reaction for diagnosis of H5N1, they said.
In another type of test, RNA isolated from exposed cells was converted to cDNA and amplified by QRT-PCR (quantitative real-time reverse transcriptase polymerase chain reaction) to quantify gene expression.
The goal of this study was to (i) measure the amount of telomerase activity in primary human urethral epithelial cells using the Telomeric Repeat Amplification Protocol (TRAP assay) (ii) evaluate the amount of methylation in the promoter of the hTERT gene by performing sodium bisulfite sequencing and (iii) assess the amount of mRNA transcribed from the hTERT gene by performing a Reverse Transcriptase Polymerase Chain Reaction (RT-PCR assay).
Researchers are working on a reverse transcriptase polymerase chain reaction test that would be as sensitive to low-titer infections and be clinically relevant for sorting out difficult cases of conflicting serology results.
Currently, the diagnostic tests including Antigen detection ELISA and real time reverse transcriptase polymerase chain reaction (RT-PCR) can be done by specialized laboratories in many countries.6
By realtime reverse transcriptase polymerase chain reaction, specific for an M gene of influenza A viruses, none of 659 duck samples and only 11 (0.9%) of 1268 geese and swan samples tested positive.
This study involved 5,067 children, of whom 919 (18%) were found by culture and/or reverse transcriptase polymerase chain reaction to have RSV infection.
Rational optimization of generic primers used for Norwalk-like virus detection by reverse transcriptase polymerase chain reaction. J Clin Virol.
Semiquantification of circulating hepatocellular carcinoma cells by reverse transcriptase polymerase chain reaction. Br J Cancer 1997;76:628-33.
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