The serum samples from both dams showed positive titre of 1:80 (160 IU) in serum agglutination test and rapid agglutination when tested against Rose Bengal Plate Test
(RBPT) antigen (Fig.8).
The seropositivity of 9.6% using Rose Bengal Plate Test (RBPT) is higher than that obtained by Ogugua et al., 2014, whose findings revealed an overall seroprevalence of 2.83% of brucellosis among goats screened across four states in Nigeria.
The seroprevalence of brucellosis in this study is lower than that reported by Al majali (27.7%) and Hamidullah et al., 2009 (34.88%), in Jordan, where positive sera with Rose Bengal Plate Test were further tested with complement fixation test for confirmation using standard Brucella abortus antigen.
Therefore, this study was carried out to evaluate diagnostic performance and accuracy of Rose Bengal Plate test (RBPT) and indirect Enzyme-Linked Immunosorbent Assay (i-ELISA) tests for screening and confirmation of bovine brucellosis using Bayesian method.
Rose Bengal Plate test (RBPT) and indirect Enzyme-Linked Immunosorbent Assay (i-ELISA) test were performed on a total of 1031 head of cattle from 82 herds (18 villages communities) owned by 52 farmers.
Rose Bengal Plate Test. RBPT was performed as described by Alton et al.
Serum samples were screened initially by Rose Bengal Plate Test
(RBPT) and then confirmed by Serum Agglutination Test (SAT) as described by Alton et al.
The humoral immunity is useful for the indirect diagnosis by all the serological tests used: Rose Bengal Plate Test, complement fixation, and ELISA .
Name of the upazila Total number RBPT +ve of samples Mymensingh Sadar 180 11 Savar 120 8 Gazipur Sadar 60 5 Total 360 24 Culture positive from Name of the upazila RBPT positive milk RBPT -ve samples Mymensingh Sadar 6 171 Savar 3 115 Gazipur Sadar 2 56 Total 11 342 Culture positive from Name of the upazila RBPT negative milk samples Mymensingh Sadar 3 Savar 2 Gazipur Sadar 1 Total 6 +ve = positive, -ve = negative, and RBPT = Rose Bengal Plate Test. Table 2: Overall seroprevalence of brucellosis in cattle.
Rose Bengal Plate Test
(RBPT), serum agglutination test (SAT) and competitive-ELISA (c-ELISA) were used as screening tests, and overall seroprevalence of brucellosis was found to be as 21%, 21% and 13% by RBPT, SAT and c-ELISA tests, respectively.
(2008) observed 4%, 3% and 3.33% seroprevalence of brucellosis in goats by Rose Bengal Plate Test
(RBPT), Serum Agglutination Test (SAT) and Milk Ring Test (MRT), respectively.