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GSNOGround-State Natural Orbital
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The efficiency of various NO donors such as S-nitrosoglutathione (GSNO), sodium nitroprusside (SNP) and CysNO in various screening experiments and their effect on seed and/or plant physiology has not been investigated so far at different pH regimes.
Different NO sources, such as SNP (sodium nitroprusside), GSNO (S-nitrosoglutathione) and CysNO are used to investigate the role of NO in plant signaling pathways.
AmB (dissolved in DMSO), L-NAME (dissolved in water), and S-nitrosoglutathione (dissolved in water) were 2-fold serially diluted across the columns and rows of a 96-well microtiter plate.
Two-way ANOVA followed by Tukey multiple comparison test was performed to analyse statistical differences between the OD measurements for treatment with different concentrations of AmB in the absence or presence of 200 mM L-NAME or 2 mM S-nitrosoglutathione. In all cases, P < 0 05 was considered statistically significant.
The BP decrease was accompanied by a sustained increase of S-nitrosoglutathione by 0.23 (0.12) nmol/L, and a dark chocolate dose resulted in the appearance of cocoa phenols in plasma.
N6022, a first-in-class inhibitor of s-nitrosoglutathione reductase (GSNOR), has the potential to be an important new treatment for acute exacerbations of asthma, chronic obstructive pulmonary disease (COPD) and inflammatory bowel disease (IBD), the company said.