When activated, mTOR phosphorylates S6K and 4E-BP1 to promote protein translation and is sensitive to rapamycin inhibition.
CD8[sup]+ Tem were sorted by flow cytometry (EPICS-XL, Beckman-Coulter, Indianapolis, IN, USA) and then stained for interferon (IFN)-a (11-7311-81, eBioscience), mTOR (ab87540, Abcam, Cambridge, MA, USA), and S6K (ab32529, Abcam) expression.
However, recent studies have established the possibility of regulation of mRNA with TOPs, independent of S6K (44).
These studies demonstrate the redundancy in the function of S6K and p90rsk in the mitogen-mediated phosphorylation of S6.
Thus, in order to delve in detail into the nutrient-sensing pathways and how they go awry in metabolic disorders, the researchers focused on an enzyme called p70 S6 Kinase 1, or S6K, which plays a role in regulating the growth and proliferation of all cells, including nerve cells.
It turns out that this enzyme, and the pathway it regulates, is nutrient sensitive - that is, S6K activity increases in the presence of carbohydrates and protein.
As shown in [Figure 4]a and [Figure 4]b, serum level of mTOR and S6K
was significantly higher in the groups of CTX-treated (99.
ACTB1003 also directly induces apoptosis by targeting the intracellular RSK and S6K
kinases at low nanomolar concentrations.
is an important downstream effector of the mammalian target of rapamycin (mTOR) and mTOR/S6K
/S6 signaling pathway is regulated in response to cytokines, nutrients and growth factors.
Inhibition of PI3K/p70 S6K
and p38 MAPK cascades increases osteoblastic differentiation induced by BMP-2.
is suppressed by the mTOR inhibitor RAD001 in SEG-1 esophageal cancer cells (Wang et al.
Deficiency or dysfunction of the encoded proteins, hamartin or tuberin, respectively, result in constitutive activation of mTOR and downstream S6K
and 4E-BP1, leading to increased protein translation and, ultimately, to inappropriate cellular proliferation, migration, and invasion.