SACPDSacramento Public Disclosure (Sacramento County, California)
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To assess the significance of the amino acid variability found in the SACPD exon 3s of Dare, other Gm SACPD exon 3s were PCR cloned and sequenced.
Thus, duplication (two copies) of each SACPD structural gene was not unexpected.
max SACPD exon 3 protein sequences derived from cDNA data suggested that soybean contains two SACPD genes (Fig.
We thought that Glycine lines-cultivars might be identified that lacked one of the SACPD genes; however, both A and B genes were found in the genomes of all 51 Glycine lines-cultivars examined (Table 1).
Abbreviations: EST, expressed sequence tag; FAD, fatty acid desaturase; RT-PCR, reverse transcription-polymerase chain reaction; SACPD, stearoyl acyl carrier protein desaturase.
The amino acid sequence of the Dare SACPDs was scanned with the program TMpred (Hoffman and Stoffel, 1993) and found not to contain transmembrane spanning sequences.
A short N-terminal transit peptide of 37 amino acids was identified for the soybean SACPDs that is presumably responsible for stroma targeting (Fig.