SASRISouth African Sugarcane Research Institute
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The financial assistance of the South African Sugarcane Research Institute (SASRI) and the South African National Research Foundation (NRF) towards this research is hereby gratefully acknowledged.
Eldana saccharina was routinely reared at SASRI using an artificial diet based on that of Gillespie (1993), which was modified from that previously described by Graham & Conlong (1988), and had ferric citrate and formaldehyde removed (Table 1).
We would like to acknowledge the staff of the Insect Rearing Unit at the South African Sugarcane Research Institute (SASRI) for their assistance in providing reared E.
Composition of the current SASRI diet for rearing Eldana saccharina.
The eggs were packaged in their labelled plastic bags and put into a cardboard box and transported back to SASRI at Mount Edgecombe, KwaZulu-Natal (S 29[degrees] 42' 24", E 31[degrees] 02' 45") by air and road.
The authors would also like to thank Nelson Muthusamy, Victor Mathonsi and the staff of the SASRI insect rearing unit for their technical support and providing material for the research.
The non-irradiated wild adults used in this study were obtained from sugarcane host plants in an area with warmer subtropical climatic conditions [Tinley Manor: long-term mean (LT[M.sub.min]) = 15.1 [degrees]C, LT[M.sub.max] = 26.2 [degrees]C, LTM = 20.7 [degrees]C; SASRI weatherweb--Tongaat-Klipfontein meteorological station], while both the irradiated and non-irradiated adults were produced under constant and controlled abiotic conditions.
saccharina strain currently produced at the SASRI insect rearing unit is suitable for use in SIT-based projects.