O loss of one in Heav'n to judge of wise, Since SATAN fell, whom follie overthrew, And now returns him from his prison scap
't, Gravely in doubt whether to hold them wise Or not, who ask what boldness brought him hither Unlicenc't from his bounds in Hell prescrib'd; So wise he judges it to fly from pain However, and to scape his punishment.
Latest studies pointed out that Foxc2 was highly expressed in developing dental tissue, including dental papilla [19-21], so it is reasonable to speculate that Foxc2 may participate in the differentiation of SCAP.
In the current research, we constructed Foxc2 and BMP2 gene-modified SCAP by lentivirus transfection technique and then investigated the synergistic effects of Foxc2 and BMP2 in inducing osteogenic and odontogenic differentiation of human SCAP.
The supernatant was used to infect the 3rd passage SCAP to obtain gene-modified cells--that is, SCAP-Foxc2 and SCAP-BMP2.
Characterization of SCAP. The papillae, as showed in Figure 1(a), were pink and kidney-shaped.
Human SCAPs were transplanted into immunocompromised mice using particles of HA/TCP as a carrier and generated a typical dentin structure.
Even more recently discovered cells as SCAP and SHED can be suggested for use in regenerative endodontics.
Volk et al., "Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papiila (SCAP)," Archives of Oral Biology, vol.
DPSCs and SCAPs were isolated according to the explant method as described previously by our group .
Scaffolds containing either 0.2% hydrogel (Puramatrix, negative control condition) or a 0.2% hydrogel suspension combined with 25,000 DPSCs and 25,000 SCAPs or a 1 : 1 mixture of DPSCs and SCAPs were subcutaneously transplanted into the dorsum of 8-week-old, female, severe combined immunodeficient (SCID) hairless outbred (SHO[TM]) mice (SHO-[Prkdc.sup.scid][Hr.sup.hr], Charles River, Wilmington, MA, USA).
Both DPSCs (Figure 2(a)) and SCAPs (Figure 2(b)) showed a pronounced expression of CD73, CD90, and CD105, while they were negative for CD31, CD34, and CD45.
Amin, "Performance analysis of copper-indium-gallium-diselenide (CIGS) solar cells with various buffer layers by SCAPS
," Current Applied Physics, vol.