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Recently, detecting SCCA is crucial in the diagnosis of cervical squamous cell carcinoma.3 For instance, Yan et al.4 reported that expressions of SCCA1 mRNA and SCCA2 mRNA functioned differently in diagnosing tumors.
In this study, the expressions of SCCA1 mRNA and SCCA2 mRNA in 76 cervical squamous cell carcinoma patients enrolled in our hospital were detected, aiming to explore their influences on the diagnosis, treatment evaluation and prognosis analysis of cervical squamous cell carcinoma.
Experimental Concept: Generally, serum SCCA2 content was tested for overall SCCA analysis.
SCCA2: upstream primer sequence: 5'-TTG CAC TTG ATC CTG TTC CAA3', downstream primer sequence: 5'-CGT ACT TAG GGG GCC CAA GGA-3'.
Peripheral bloods were collected, and the SCCA2 contents were detected by SCCA2 ELISA kit, with the values Greater than 1.5 mg/L being abnormally expressed.
Table-I: Relative expression rates of SCCA1 and SCCA2 before treatment.
Relative Expression Rates of SCCA1 and SCCA2 before Treatment: The two groups had similar relative SCCA1 expression rates (P Greater than 0.05), whereas the relative SCCA2 expression rate of the study group (4.396+-2.002) was significantly (P=0.0000982 Less than 0.05) lower than that of the control group (9.028+-3.182) (Table-I and Fig.2).
Relative SCCA2 Expression Rate and Correlated Factors: Relative SCCA2 expression was significantly correlated with lymphatic metastasis, T classification and clinical stage.
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- SCCA2/SCCA1 fusion protein