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Characterization of SCCmec elements in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures from neonates during three decades.
Identification of the multiresistant and non-multiresistant SCCmec types of MRSA, heterogeneity of CHDL-encoding genes of CRAB, OXA-23-type as the major carbapenemase mechanism, and the presence of CTX-M-55 of CTX-M [beta]-lactamases would aid in research and development priorities globally.
A prevalence of SCCmec types IV and V, typically from the community, were reported in all CA-MRSA isolates, and were the main types found among HA-MRSA isolates (63%) as well.
Metisilin direncine neden olan penisilin baglayan protein 2a (PBP2a), SCCmec gen kaseti uzerinde bulunan mecA geni tarafindan kodlanmaktadir ve bu SCCmec tip IV'un boyutunun kucuk olmasi nedeniyle bakteriyel yayilim daha kolay olmaktadir (8).
aureus strain Culture level of detection method 1 2 Reference ATCC 25923 Direct Plate [10.sup.2] [10.sup.2] MSSA, SCCmec Static BE 10 [10.sup.2] negative Agitated BE 10 [10.sup.2] Reference ATCC 43300 Direct Plate [10.sup.2] [10.sup.2] MRSA, SCCmec type IV Static BE [10.sup.2] 10 Agitated BE [10.sup.2] [10.sup.2] Clinical infecting Direct Plate [10.sup.2] [10.sup.3] isolate Static BE [10.sup.2] [10.sup.3] MRSA, SCCmec Type IV Agitated BE [10.sup.2] [10.sup.2] Clinical colonizing Direct Plate [10.sup.3] [10.sup.2] isolate Static BE [10.sup.3] [10.sup.2] MRSA, SCCmec Type II Agitated BE 10 [10.sup.2] Clinical colonizing Direct Plate [10.sup.2] [10.sup.2] isolate Static BE 10 [10.sup.3] MSSA, SCCmec Type IV Agitated BE 10 [10.sup.2] Replicate, lowest S.
aureus WIS were used as reference strains for SCCmec typing.
SCCmec typing was carried out using established specific primers for amplification of mec, ccr, and J region of staphylococcal cassette chromosome mec as described by Ghaznavi-Rad et al.
They have 99.8%, 80% and 91% nucleotide identities, respectively, to the mecA gene carried by SCCmec on the MRSA chromosome.
El motivo de la resistencia a la meticilina y a los demas betalactamicos es la presencia del gen mecA en la bacteria, especificamente en el elemento cromosomico movil denominado Staphylococcal Cassette Chromosome mec (SCCmec).
(7) This gene is contained in a chromosome cassette of 21 to 60 kb called SCCmec, a mobile genetic element that may also contain genetic structures, such as Tn554, pUB110, pT181.
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