SCGESingle Cell Gel Electrophoresis
SCGESpatial Computable General Equilibrium (model)
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Abbreviations: iTRAQ, isobaric tags for relative and absolute quantitation; MTT, 3-(4, 5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide; AO, acridine orange; EB, ethidium bromide; DAPI, 4,6-diamidino-2-phenylindole; SCGE, single cell gel electrophoresis assay; TEM, transmission electron microscopy; ROS.
The extent and distribution of DNA damage indicated by the SCGE assay was evaluated by examining at least 200 randomly selected and non-overlapping cells on the slides per each animal.
The data obtained from the micronucleus and SCGE assays were submitted to One-way analysis of variance test (ANOVA) and the Tukey-Kramer multiple comparison test (Sokal and Rohlf 1995), using the GraphPad Instat [R] software (version 3.
5 ml of blood was collected from retro-orbital sinus, and was mixed with double amount of phosphate buffer saline (PBS) and was processed for SCGE (comet assay) (9).
The SCGE data were transferred into Microsoft Excel (Japanese Windows 98 version; Microsoft Corporation, Tokyo, Japan) and pooled from the repeated experiments.