B = BsrgI, Sc = ScrFI
, M = MspI, Ha = HaeIII, Hh = HhaI, Dp = DpnI, Hi = HinFI, Dd = DdeI, Ta = TaqI, Rs = RsaI, Sa = Sau96A; + = homozygous presence of restriction site, - = homozygous absence of restriction site, H = heterozygote at a site.
Furthermore, the PCR products were digested in 37[degrees]C stable incubation by distinguished restriction enzyme MboII (New England Biolabs, Peking, China) for 30 minutes of rs4758680 and ScrFI
(New England Biolabs, Peking, China) for 2 hours of rs7977932 as shown in Table 1, separately.
[TABULAR DATA FOR TABLE 2 OMITTED] RFLP haplotypes were determined by separate digestion of each PCR product with five restriction enzymes (RsaI, HinFI, DdeI, ScrFI
, and NlaIII) with four-base recognition sequences and one restriction enzyme (AseI) with a six-base recognition sequence, followed by ethidium-bromide visualization in 2% agarose gels containing 1X TBE buffer.