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The number of cells isolated from the SDLN was altered by vitamin [D.sub.3] supplementation of female mice.
CD4+CD25+(Foxp3+) cells from the SDLN (Figure 4(a)), MLN (Figure 4(c)), spleen (Figure 4(d)), and blood (Figure 4(e)) of vitamin [D.sub.3]-replete mice had increased capacity to suppress IL-2 production by cocultured responder cells.
We examined the expression of neuropilin on [T.sub.Reg] cells from the skin, SDLN, lungs, or ADLN of vitamin [D.sub.3]-replete or vitamin [D.sub.3]-deficient mice and observed no difference in the expression of this molecule (Figure 5(a)).
Dietary Vitamin [D.sub.3] Did Not Affect the Expression of CCR4 or CCR10 on Cells in the SDLN or ADLN.
Dietary Vitamin [D.sub.3] Increased the Percentages and Activity of [T.sub.Reg] Cells in the SDLN. We observed a greater suppressive activity of CD4+CD25+(Foxp3+) cells isolated from the SDLN, MLN, spleen, and blood of mice fed the vitamin D-containing diet, suggesting a systemic effect of dietary vitamin D on [T.sub.Reg] cell activity.
Our studies suggest that dietary vitamin [D.sub.3] increased the percentages and suppressive function of [T.sub.Reg] cells in the SDLN and that these cells are poised to suppress dermal inflammation.
1,25[(OH).sub.2][D.sub.3]: 1,25-Dihydroxyvitamin [D.sub.3] 25(OH)[D.sub.3]: 25-Hydroxyvitamin [D.sub.3] ADLN: Airway-draining lymph nodes DNFB: 2,4-Dinitrofluorobenzene [T.sub.Eff] cells: Effector T cells MLN: Mesenteric lymph nodes [T.sub.Reg] cells: Regulatory T cells SDLN: Skin-draining lymph nodes VDBP: Vitamin D-binding protein.
Caption: Figure 2: Dietary vitamin [D.sub.3] increased the percentage of Foxp3+ [T.sub.Reg] cells in the SDLN. Female offspring born to vitamin [D.sub.3] - (vitD-) replete (+) or vitamin [D.sub.3]-deficient (-) BALB/c mothers were maintained on the vitamin [D.sub.3]-replete or vitamin [D.sub.3]-deficient diets (resp.).
Figure 3: Dietary vitamin [D.sub.3] reduced the absolute number of cells in the SDLN. Female offspring born to vitamin [D.sub.3]- (vitD-) replete (+) or vitamin [D.sub.3]-deficient (-) BALB/c mothers were maintained on the vitamin [D.sub.3]-replete or vitamin [D.sub.3]-deficient diets (resp.).
CD4+CD25+(Foxp3+) cells were purified from (a) SDLN, (b) ADLN, (c) MLN, (d) spleens, or (e) blood of mice and cocultured with lymph node responder cells from D011.10 mice at ratios of 1: 8,1: 16, or 1: 32 and OVA peptide.
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